Ultrastructural distribution of basic fibroblast growth factor-like molecules in peripheral nerves
CitationKayton, R. J. ve Aktaş, R. G. (1998). Ultrastructural distribution of basic fibroblast growth factor-like molecules in peripheral nerves. Microscopy and Microanalysis, Cambridge University Press. 4(2), s. 1102-1103.
Basic Fibroblast Growth Factor (bFGF) is a multifunctional polypeptide which has been shown to play a pivotal role in the survival and differentiation of nerve cells. Several trophic and non-trophic functions of this protein have been suggested in peripheral nerves. In spite of ample information about the distribution and effects of bFGF in central nervous system, few data are available concerning the localization of this protein in peripheral nerves. In view of the role of bFGF in regulation of trophic and non-trophic functions, we particularly focused on the presence and precise location of bFGF in peripheral nerves at the electron microscope level. Spurr's resin embedded ultrathin sections from adult rats’ sural nerves were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-Zymogenetics) specific for bFGF using two-step immunogold labeling method. Control samples were treated with either an equivalent volume of blocking solution (omitting the primary antibody) or an irrelevant antibody (Factor VIII, VGF, anti-histamine, anti-fibroblast 5B5).
SourceMicroscopy and Microanalysis
- Makale Koleksiyonu 
The following license files are associated with this item: