Electron microscopic ımmunolocalization of basic fibroblast growth factor-like molecules in capillary endothelial cells
CitationKayton, R. J. ve Aktaş, R. G. (1998). Electron microscopic ımmunolocalization of basic fibroblast growth factor-like molecules in capillary endothelial cells. Microscopy and Microanalysis, Cambridge University Press. 4(2), s. 1100-1101.
Basic fibroblast growth factor (bFGF) is a potent angiogenic polypeptide. It promotes angiogenesis in vivo and in vitro by stimulating migration, proliferation and proteolytic activity of endothelial cells. Whereas several effects of exogenous bFGF on endothelial cells have been described, it has remained unclear how endogenous bFGF produced by vascular endothelial cells regulate angiogenesis. To further investigate functional implications of the distribution of bFGF, we undertook the present study. Our aims were (i) to identify the specific location of bFGF in endothelial cells using electron microscopy immunogold labeling technique (ii) to determine the distribution of bFGF in capillaries of different types of tissues. Tissue samples from sciatic nerve, hippocampus, adrenal gland and kidney of normal adult rats were fixed in 4% paraformaldehyde/1 to 5% glutaraldehyde and embedded in Spurr's resin. Ultrathin sections were labeled with either polyclonal (F3393-Sigma) or monoclonal antibodies (F6162-Sigma, C3316-ZymoGenetics) specific for bFGF using a two-step immunogold labeling method.
SourceMicroscopy and Microanalysis
- Makale Koleksiyonu 
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