Evaluation of the in-vitro effects of collagen supplements on liver cancer
AuthorKahveci Ulugöl, Reyhan
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CitationTüylü, H., Kahveci Ulugöl, R. ve Gülhan Aktaş, R. (2020). Evaluation of the in-vitro effects of collagen supplements on liver cancer. 4th Internatıonal Medical Student Congress of Bucharest, The Medical Students’ Society of Bucharest. s. 42-43.
Introduction: Hepatocellular carcinoma (HCC) is the 5th most common cancer in the world and in the 3rd place of cancer related deaths. The effects of microenvironment significantly affect the behaviour of cancer cells. Type I and Type III collagen is commonly used in food supplements. The current study aims to investigate the in-vitro effects of hydrolyzed collagen that is used as food supplement on liver cancer. Methods: HepG2 cell line was obtained from ATCC. The cells were divided into 5 experimental groups: (I) Control, (II) cells on type I collagen coated surfaces (III) cells on type III collagen coated surfaces, (IV) cells on Type I+ Type III collagen coated surfaces, (V) and (VI) cells that were fed with Type I and Type III collagen containing food supplement at low and high concentration. Live images of the cells were taken under phase contrast microscope at 24 th,48 th,72 th,96 th and 120 th hours. The cells were xed with 4% formaldehydeIn after 24 and 120 hours, stained with Hematoxylin & Eosin; or immuno-labeled with AFP and OV6 antibodies. EROD method was used to investigate Cytochrome P450 1A1 enzyme activity. Data was evaluated by using SPSS. Results: Expression of AFP and OV6 were increased in group II and III. In contrast, decrease at AFP and Ov6 levels was clear in V and VI group. The increase at the EROD activity in the food supplement-added groups was evident. Conclusion: The results show the different effects of Type I and Type III collagen in various microenvironmental conditions. Carcinogenic activity in the cells on coated surfaces increase, while the collagen in food supplements decrease this carcinogenic activity. Further studies are needed to clarify the mechanisms of those effects on cancer cells.
Source4th Internatıonal Medical Student Congress of Bucharest
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