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Yayın An immunoelectron microscopic study: helix pomatia lectin gold complex in breast cancer(Gazi Üniversitesi, 1996) Şeftalioğlu, Aysel; Alabay, Belma; Özcan, Ziya; Cıncık, Mehmet; Kendir, BanuAbnormal cellular glycolysation demonstrated by the bindings of HElix Pomatia Lectin (HPL) to paraffin-embedded sections has been shown in several studies to be associated with aggressive biological behavior and poor longterm prognosis in breast cancer. We investigated the relation between HPL staining of primary infiltrative duetal breast cancer and the presence of axillary lymph node (AX) metastases at the ultrastructured level by means of postembedding labeling. Breast cancer tissue fragments were fixed mild glutaraldehyde and embedded in Agar resin 100. Helix Pomatia Lectin Gold complex (HPL-GC) was chosen as a marker. A satisfactory labeling and fine structure preservation were obtained. Dense HPL binding sites were observed over the plasma membrane and the eytoplasm of cancer cells. Absorbing lectin with appropriate sugar established the specificity of binding and allowed HPL-GC to be used as a sensitive and specific reagent. The ersults of this study suggest that expression of HPL binding sites in breast cancer cells is of prognostic signifiance and may reflect the ability of tumor to invade and metastasize.