Gökçeoğlu Kayalı, DamlaÇavdaroğlu, SudeAktaş, Ranan GülhanKorhan, Betül EdaRoxas, Mark Joshua D.Kıdıryuz, Merve2024-07-122024-07-122020Gökçeoğlu Kayalı, D., Çavdaroğlu, S., Aktaş, R.G. ve Korhan, B. (2020). Effects of high dose insulin on cell proliferation and alpha feto protein expression in human liver cancer cell line models. Roxas, M.J.D. ve Kıdıryuz, M. (Ed.). 3rd International Biltek Conference On Science, Technology & Current Developments In Social Sciences, Institute of Economic Development and Social Researches. s. 122-133.978-625-7139-10-6https://hdl.handle.net/20.500.12415/4572Introduction: The relationship between the development of liver cancer and the insulin / IGF signal pathway has been recently demonstrated. There are also studies that points out increase in several cancer types due to usage of insulin at diabetic patients. In our study, we aimed to find the answers to the following questions: (i) How do the stimulation of insulin receptors with high-dose insulin affect cell viability and Alpha-feto Protein (AFP) expression- one of the most important tumor markers- in liver cancer? (ii) Does interaction between the liver cancer cells and type 1 collagen on basement membrane in the liver effect the response of cancer cell to high dosage insulin? Method: A human liver cancer cell line, HepG2 cells were divided into 4 groups: (i) Classic cell culture group, (ii) Classic cell culture on type 1 collagen coated surfaces, (iii)High-dose insulin administered group in classic cell culture, (iv) High-dose insulin administered group on type 1 collagen coated surfaces . High-dose of insulin was determined as 10-7 mol/L and incubated 24 hours. To assess cell viability, MTT was performed. Cells were marked by using indirect immunohistochemistry methods to inspect AFP expression and then they were examined under a confocal microscope to demonstrate the location of AFP three-dimensionally. AFP expression levels manifested by the Zen scanning analysis system and MTT ratios were correlated by using one-sided ANOVA and Tukey/Bonferroni post Hoc tests. Result: In high-dose insulin groups there was a meaningful decrease both in non-collagen (p< 0.01) and collagen control (p< 0.01) in terms of cell viability. High-dose insulin caused significant decrease at the cell viability in non-collagen control group (p< 0.01). AFP expression decreased significantly in both non-collagen and collagen coated and high dosage insulin applied groups were when compared to non-collagen (p< 0.01) and collagen (p< 0.01) control Discussion: High dose insulin treatment caused significant change at the cancer cell viability both with and without the effect of Type I collagen. In contrast, AFP expression decreased in those groups. Signalling between liver cancer cells and Type I collagen did not alter AFP expression levels. The results of the study reveals that usage of insulin by diabetic patients might affect AFP levels, which is one of the most important liver cancer marker. This change should be considered during the diagnosis, prognosis and treatment of liver cancer. . (Note: This research is being founded by TÜBİTAK. Project No: 1919B011900393 )trinfo:eu-repo/semantics/openAccessHepatocellular carcinomainsulin resistanceinsulin receptorsAlpha-feto ProteinHepG2Conference Object133122