Öner, ÇağrıSoyergin, DilaraÖzyurt, AhmetÇolak, Ertuğrul2024-07-122024-07-122022Öner, Ç., Soyergin, D., Özyurt, A. ve Çolak, E. (2022). 4-hydroxycoumarin effects on both cellular and genetic characteristics of hepatocellular carcinoma cells. Cytology and Genetics, Springerlink. 56, s. 292-300.1934-944010.3103/S00954527220300942-s2.0-85132278087https://link.springer.com/article/10.3103/S0095452722030094https://doi.prg/10.3103/S0095452722030094https://hdl.handle.net/20.500.12415/36414-Hydroxycoumarin is an aromatic substance which is metabolized in liver and used as a therapeutic agent for various diseases. We aimed to determine the impact of 4-Hydroxycoumarin on HepG2 cells according to their viability, proliferation, adhesion and gene expression of cellular behavior parameters. Inhibitory concentration 50 (IC50) of 4-Hydroxycoumarin was detected on HepG2 cells. After determining the optimal time and concentration, the effect of 4-Hydroxycoumarin on viability, proliferation and adhesion of HepG2 cells were observed. Gene expressions of Ki-67, MMP-2, MMP-9 and piR-823 expression were determined by using Real Time-Polymerase Chain Reaction. IC50 value of 4-Hydroxycoumarin on HepG2 cells was 5 ?M at the 48 h (p < 0.001). 5 ?M at the 48 h of 4-Hydroxycoumarin caused to decrease of proliferation (p < 0.001) and viability of HepG2 cells (p < 0.001). Viability rate were supported by hematoxylin-eosin staining. Adhesion of cells increased on 4-Hydroxycoumarin treated cells compared to control (p < 0.001). While Ki-67 gene expression of 4-Hydroxycoumarin treated group decreased (p < 0.001); upregulation of MMP-2, MMP-9 and piR-823 expressions were determined in 4-Hydroxycoumarin treated group (p < 0.001). According to the cellular and genetic perspective, 4-Hydroxycoumarin might be useful to treat hepatocellular carcinoma. High adhesion and proliferation are the main characteristics of HepG2 cells, 4-Hydroxycoumarin treatment caused to lose these functions. The genetic markers of these characteristics also supported the same result. These are first findings about the effect of 4-Hydroxycoumarin on piR-823 and genes which are key features of cellular survival mechanisms.eninfo:eu-repo/semantics/openAccess4-Hydroxycoumarinmatrix metallo proteinasepiRNApiR-823hepatocellular carcinomaArticle300Q429256WOS:000812257100011Q4