Ates, BerenOner, CagriAkbulut, ZeynepColak, Ertugrul2024-07-122024-07-1220232251-96372251-964510.22088/IJMCM.BUMS.11.3.2362-s2.0-85170266187https://doi.org/10.22088/IJMCM.BUMS.11.3.236https://hdl.handle.net/20.500.12415/6908Capsaicin is a natural product which is extracted from pepper and has the potential to be used in cancer treatment because of its anti- proliferative effects. The aim of the study was to determine the effect of capsaicin on the hepatocellular carcinoma cell proliferation and the expressions of related genetic markers as Ki-67, PI3K/AKT/mTOR and epigenetic markers as miR-126 and piR-Hep-1. The inhibitory concentration of capsaicin in HepG2 cells was determined. piR-Hep-1 and miR-126 expressions and Ki-67, PI3K, AKT and mTOR gene expressions were examined by RT-PCR. The inhibitory concentration of capsaicin for HepG2 cells was 200 nM and the decreased proliferation was observed at 24th hour. As epigenetic markers, an up regulation of miR-126 and down regulation of piR-Hep-1 expression were determined after treatment. Moreover, Ki-67, PI3K and mTOR gene expressions decreased while AKT gene expression increased after the treatment (p<0.001). According to the obtained data, capsaicin has an impact on proliferation both genetically and epigenetically. Furthermore, treatment of capsaicin effects miR-126 and piR-Hep-1 expressions which effect carcinogenesis in different way. Moreover, there are some clues which indicate that these two small non-coding RNA might affect each other and share the same target molecules post-transcriptionally.eninfo:eu-repo/semantics/closedAccessCapsaicinMir-126Pir-Hep-1Pi3k/Akt/Mtor SignallingArticle243337605741Q323611WOS:001070823800005N/A