Turgut Coşan, DidemSoyocak, AhuÖner, Çağrı2024-07-122024-07-122020Turgut Coşan, D., Soyocak, A. ve Öner, Ç. (2020). Inhibition of voltage?gated potassium channels affect expressions of miR-126 and miR-126* in breast cancer cell lines. Bratislava Medical Journal, AEPress. 121(2), s. 111-116.1336-034510.4149/BLL_2020_0142-s2.0-85080840671http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=6525&category_id=158&option=com_virtuemart&vmcchk=1&Itemid=1https://doi.prg/10.4149/BLL_2020_014https://hdl.handle.net/20.500.12415/3592AIM: We aimed to determine the possible correlation between voltage?gated potassium channels and micro RNAs in breast cancer and metastatic breast cancer cells. METHOD: Kv1.3 and Kv10.1 channels were inhibited by specific siRNAs using a lipofectamine-based transfection in MCF-7 and MDA-MB-231 cells. After transfection, total RNA was isolated, and then miR-126 and miR-126* expressions were observed using RT-PCR. RESULTS: There was a negative correlation between Kv channels and miRNAs according to the characteristics of the breast cancer cells. The inhibition was observed not only in Kv1.3 but also in Kv10.1 in MCF-7 cells, and miR-126 and miR-126* expressions were downregulated compared to the control group (p < 0.001). The inhibition of these channels in MDA-MB-231 cells caused an upregulation of miR-126 and miR-126* expressions (p < 0.001). CONCLUSION: The miR-126 and miR-126* expressions differed according to benign and malign breast cancer cell lines. Furthermore, we found that miR-126/126* may interact with Kv1.3 and Kv10.1 voltage-gated potassium channels. Our study suggests and indicates the relationship between Kv channels and miRNAs in breast cancer cells (Tab. 1, Fig. 2, Ref. 51).eninfo:eu-repo/semantics/openAccessbreast cancerKv1.3Kv10.1miR-126miR-126*Article116232115962N/A111121WOS:000508930100002Q3