Yazar "Burgut, Hüseyin Refik" seçeneğine göre listele
Listeleniyor 1 - 20 / 40
Sayfa Başına Sonuç
Sıralama seçenekleri
Yayın Accumulation of p53 protein in astrocytomas: immunohistochemical study using D07 MAb(Novartis Onkoloji, 1995) Gönlüşen, Gülfiliz; Zorludemir, Suzan; Coşar, Ediz; Kaya Selçuk, Fazilet; Burgut, Hüseyin Refik; Tuncer, İlhanAccumulation of p53 protein was examined in tissue sections of 40 astrocytomas and compared with the histological grade of tumor, age and sex of the patients. lmmunohistochemical staining with monoclonal antibody (D07) was positive in 15.4% of grade II and 45% of grade lll-IV astrocytoma cases; whereas none of the pilocytic astrocytomas (grade I) were positive for p53. lmmunoreactivity showed a strong correlation with tumor grade. There was no significant difference between age and sex of p53 positive and negative cases.Yayın Accuracy of bedside diagnosis versus allen and siriraj stroke scores in Turkish patients(Wiley Online Library, 2006) Özeren, Ali; Bıçakcı, Şebnem; Burgut, Hüseyin Refik; Sarıca, Yakup; Bozdemir, HacerComputerized tomography and magnetic resonance imaging allow the accurate diagnosis in stroke and distinction of ischemic from hemorrhagic lesions. However, clinical diagnosis is still critical where neuroimaging techniques are not available, especially to establish first?aid measures in a stroke patient. In this prospective study of 300 patients with stroke, the diagnosis of ischemic and hemorrhagic strokes was made as an informal bedside diagnosis prior to neuroimaging. The accuracy of bedside diagnosis was also compared with the results of the Allen and Siriraj stroke scores. Then, the reliability of these scores and our informal bedside diagnosis, along with the final diagnosis, were compared with each other. Our informal bedside diagnosis was correct in 250 of the 300 patients (83.3%). The diagnostic sensitivity and positive predictive value (PPV) of bedside diagnosis for ischemic stroke were 87.8% and 86.0% and for intracerebral hemorrhage 75.7% and 78.5%, respectively. Sensitivity and PPV for every cut?off value of the Allen and Siriraj stroke scores were less than that of sensitivity and PPV of informal bedside diagnosis. When the receiver operating curves obtained from the Allen and Siriraj stroke scores were compared, the Allen stroke score was found to be a better predictor in the final diagnosis. Our data suggest that an informal bedside diagnoses is as good as diagnoses made on certain intracerebral hemorrhages and on certain ischemic strokes by the Siriraj and Allen stroke scores; when compared, the Allen score seems to be better than the Siriraj stroke score. Hence, the use of both scores is recommended in epidemiologic studies as a screening tool as suggested by previous studies employing other diagnostic tools in clinics.Yayın Adjuvant use of antiprolactin, antiestrogen, and cytotoxic chemotherapy for breast cancer(Elsevier, 1994) Erkişi, Melek; Burgut, Hüseyin Refik; Ünsal, Mustafa; İspir, Turgay; Varinli, Seyhan; Kılıçoğlu, RemziBetween September 1, 1985 and September 1, 1989, 110 premenopausal patients with estrogen-receptor-positive, stage-II breast cancer were randomized to receive, as adjuvant treatment following radiotherapy, either cyclophosphamide, methotrexate, and 5-fluorouracil (CMF) + tamoxifen (T) + bromocriptine (B) bases or CMF + T only. Preoperative serum prolactin (PRL) levels or PRL-receptor status of the tumor were not available, but before the commencement of adjuvant therapy serum PRL levels were measured in all patients and found to be high in 28. The local (LR) and distant (DM) metastasis-recurrence rates were lower in patients given bromocriptine (CMF + T + B) (LR, 5.7%; DM, 10.9%) than in those not given bromocriptine (CMF + T) (LR, 10.9%; DM, 27.2%); these findings, however, were not significant (P > 0.05). In the 28 hyperprolactinemic patients the metastasis-recurrence rate (17/28) was higher (P = 0.0001) and disease-free survival was shorter (P = 0.001) than in the 80 normoprolactinemic patients. It was also demonstrated that the disease-free survival was longer (P = 0.009) and the metastasis-recurrence rate was lower (6/12) in hyperprolactinemic patients who received bromocriptine (CMF + T + B) than in hyperprolactinemic patients who did not (CMF + T) (11/16), while there was no difference in metastasis-recurrence rates and disease-free survival between the two treatment groups among normoprolactinemic patients. These results encourage further investigation of the action of bromocriptine adjuvant base. Baseline serum PRL measurement and tumor PRL-receptor determination could be valuable tools to identify the appropriate cases for antiprolactinemic treatment.Yayın Alpha-fetoprotein and carcinoembryonic antigen in gastric carcinoma(Springer Nature, 1994) Melek, Erkişi; Figen, Doran; Burgut, Hüseyin Refik; Salih, Çolakoğlu; Karaer, P.; Varinli, SeyhanFifty-four early stage gastric cancer patients were investigated for the presence of AFP and CEA. In their tumor specimens 9 patients showed AFP, 14 patients showed CEA and 9 patients showed AFP and CEA positivity by immunoperoxidase staining. Mean age was younger in AFP (+) patients (44.35 ± 8.4) than others (53.5 ± 8.0, p=0.0006). There was a significant correlation between the AFP and CEA positivity and stage of the disease (p=0.001, p=0.001). Lymphatic and/or venous invasion were more prominent in tumors with positive AFP staining (p=0.04) but serosal invasion was more frequent in CEA (+) tumors (p=0.05). The incidence of poorly differentiated tumors was high in AFP (+) tumors (p=0.034). Disease free survival (DFS) and overall survival (OS) were significantly shorter in patients with high serum AFP and CEA levels (p=0.0001). As conclusion AFP and CEA content of the tumor is a very important parameter in the management and prognosis of gastric cancer patients.Yayın Anti?HCV and HCV?RNA prevalence and clinical correlations in cases with non?Hodgkin's lymphoma(Wiley Online Library, 2003) Paydaş, Semra; Kılıç, Banu; Yavuz, Sinan; Disel, Umut; Tanrıverdi, Kahraman; Şahin, Berksoy; Burgut, Hüseyin RefikHepatitis C virus (HCV) is an RNA virus in the Flaviviridae family. It displays lymphotropism in addition to hepatotropism and extrahepatic manifestations are very well known. There are many studies showing an association between HCV infection and non?Hodgkin's lymphomas (NHL). In this study the evidence for HCV infection was studied in cases with NHL. To this end, anti?HCV antibody and HCV?RNA were screened in serum samples of cases with NHL using third?generation ELISA and RT?PCR. Anti?HCV antibody was studied in 223 patients and was found to be positive in 18 cases (8.1%). Anti?HCV antibody positivity was compared with our blood bank / blood donor population. There was an important increased risk of HCV infection—the common odds ratio was 34.56 and corrected odds ratio was 19.07. HCV?RNA was studied in 67 of 223 serum samples. HCV?RNA was found to be positive in 21 of 67 samples (31.3%). When compared with clinico?demographic parameters for anti?HCV and HCV?RNA, including age, nodal status, and grade (in evaluable cases), except age in cases with or without HCV?RNA, we did not find an important correlation with HCV status and clinical findings (P = 0.155; 0.442; 0.288 for anti?HCV and 0.027; 0,558; 0.126, respectively). These results suggest that HCV infection may be an important risk factor for lymphomagenesis and HCV?RNA is more useful for the detection of HCV infection in these immunosuppressed cases. Simultaneous detection of anti?HCV and HCV?RNA will be more informative in this population.Yayın Comparing the methods of measuring multi-rater agreement on an ordinal rating scale: a simulation study with an application to real data(Taylor and Francis Online, 2013) Sertdemir, Yaşar; Burgut, Hüseyin Refik; Alparslan, Zeliha Nazan; Ünal, İlker; Günaştı, SuhanAgreement among raters is an important issue in medicine, as well as in education and psychology. The agreement among two raters on a nominal or ordinal rating scale has been investigated in many articles. The multi-rater case with normally distributed ratings has also been explored at length. However, there is a lack of research on multiple raters using an ordinal rating scale. In this simulation study, several methods were compared with analyze rater agreement. The special case that was focused on was the multi-rater case using a bounded ordinal rating scale. The proposed methods for agreement were compared within different settings. Three main ordinal data simulation settings were used (normal, skewed and shifted data). In addition, the proposed methods were applied to a real data set from dermatology. The simulation results showed that the Kendall’s W and mean gamma highly overestimated the agreement in data sets with shifts in data. ICC4 for bounded data should be avoided in agreement studies with rating scales <5, where this method highly overestimated the simulated agreement. The difference in bias for all methods under study, except the mean gamma and Kendall’s W, decreased as the rating scale increased. The bias of ICC3 was consistent and small for nearly all simulation settings except the low agreement setting in the shifted data set. Researchers should be careful in selecting agreement methods, especially if shifts in ratings between raters exist and may apply more than one method before any conclusions are made.Yayın Detection of hepatitis C virus RNA in paraffin-embedded tissues from patients with non-Hodgkin's lymphoma(National Center for Biotechnology Information, 2004) Paydaş, Semra; Ergin, Melek; Tanrıverdi, Kahraman; Yavuz, Sinan; Disel, Umut; Kılıç, Nil Banu; Erdoğan, Şeyda; Şahin, Berksoy; Tunçer, İlhan; Burgut, Hüseyin RefikThe aim of this study is to detect the possible role of hepatitis C Virus (HCV) in lymphomagenesis. HCV-RNA and anti-HCV antibodies were studied in tissue and serum samples taken from patients with non-Hodgkin's Lymphoma (NHL). The prevalence of HCV, the clinical presentation of these cases, and association with histologic subtypes were determined. RT-PCR was used to detect the HCV-RNA in serum and tissue samples. The anti-HCV antibodies were tested with microparticle enzyme immunoassay. Immunohistochemistry with the ABC method was used to detect the HCV core protein in HCV-RNA(+) cases. RNA could be detected in 30 of 35 cases, and other tests were performed in these 30 samples. HCV-RNA was detected in 11 tissue samples (11/30, 37%). HCV core protein was studied in 10 of 11 HCV-RNA(+) cases, and 1-3% nuclear staining was found in only 2 samples. Serologically, HCV-RNA was detected in 7 of 30 samples (23.3%) and anti-HCV antibody was detected in 3 of 30 samples (10%). Detection of HCV-RNA in 37% of the lymphoma tissue samples suggests that HCV may have a role or is a contributing factor in the pathogenesis of lymphoma. The very low HCV core protein in lymphoma tissues may be due to the low viral load in lymphoid tissues and/or higher sensitivity of the PCR method. Detection of anti-HCV antibody in only three cases may be associated with undetectable levels of antibodies due to the immune deficiency in cases with NHL.Yayın Determinants of road traffic injuries in drivers in a rapid highly economically developing country: a major global public health crisis(BMJ Publishing Group, 2010) Bener, Abdulbari; Burgut, Hüseyin Refik; Sidahmed, Heba; Albuz, Rama; Sanya, Rahima; Khan, Waleed AliAim The objective of this study was to determine the characteristics of road traffic injuries (RTIs) among Qatari drivers and examine the human behavioural and environmental risk factors associated in occurrence of RTIs. Design This is a cross sectional survey. Setting Primary Healthcare Centers in the State of Qatar. Methods This study was conducted during the period of February–July 2009. A random sample of 1800 Qatari drivers was approached and 1406 drivers responded and agreed to participate in this study, with a response rate of 78.1%. Face to face interview was conducted by well-trained research assistants based on a questionnaire covering socio-demographic information, driving history, type of vehicle, driver behaviour, severity of injuries and nature of injuries. Results The study revealed that of the studied Qatari drivers (1406), 14.5% of them were injured. Young drivers in the age group 25–34 years were more involved in RTIs (35.8%). The RTIs occurred more among male drivers than females with the ratio 1.7:1 (p<0.001). Overall, 53.9% of the studied Qatari drivers were injured from traffic violations, especially with exceeding speed limit (25.9%) and parking violation (18.1%). 28.4% of the injured drivers were distracted with eating or drinking and 25% using mobile phone. Qatari drivers were more injured from overturn skid crashes (20.6%) and hitting fixed objects (14.7%). Head injuries were reported more from light vehicle crashes (33.3%) and neck injuries from Pickup and SUVs crashes (44.7%). Severe injuries were reported among Qatari drivers who had heavy vehicle crashes (43.5%). Conclusion The study findings revealed the high risk of RTIs was among young male Qatari drivers in the age group (25–34) years. It showed that human behavioural factors represent one of the main causes of RTIs such as excessive speed and using seatbelt. Head and neck injuries were reported more among Qatari drivers.Yayın DNA ploidy, proliferative activity, and concanavalin A reactivity in breast cancer(Wiley Online Library, 1994) Paydaş, Semra; Sarpel, Süleyman; Gilman Sachs, Alice; Tuncer, İlhan; Pehlivan, Sacide; Tunalı, Nurdan; Zorludemir, Suzan; Burgut, Hüseyin Refik; Küçük, ÖmerParaffin?embedded primary tumor specimens from 48 patients with breast cancer were examined for DNA ploidy, S?phase fraction (SPF), and concanavalin A (Con A) reactivity. The results were correlated with clinicopathological prognostic factors, including patients' age and menopausal status, stage of disease, nuclear grade, and size of the primary tumor. There were no associations among ploidy, SPF, Con A reactivity, and menopausal status, stage of disease, or size of the primary tumor. However, among patients who were 50 years or older, 81 % had diploid tumors and 73% had good reactivity (3+ or better staining score) with Con A. In contrast, among patients who were younger than 50 years, 45% had diploid tumors (P < 0.05) and 21% had good Con A reactivity (P < 0.05). Seven of 19 (37%) poorly differentiated tumors and 7 of 9 (78%) moderately differentiated tumors had good reactivity with Con A (P < 0.05). Reactivity of tumor cells with Con A in primary breast cancer tissues deserves further evaluation as a potential biomarker of prognosis. © 1994 Wiley?Liss, Inc.Yayın Does the decision in a validation process of a surrogate endpoint change with level of significance of treatment effect? A proposal on validation of surrogate endpoints(National Center for Biotechnology Information, 2009) Sertdemir, Yaşar; Burgut, Hüseyin RefikBackground: In recent years the use of surrogate end points (S) has become an interesting issue. In clinical trials, it is important to get treatment outcomes as early as possible. For this reason there is a need for surrogate endpoints (S) which are measured earlier than the true endpoint (T). However, before a surrogate endpoint can be used it must be validated. For a candidate surrogate endpoint, for example time to recurrence, the validation result may change dramatically between clinical trials. The aim of this study is to show how the validation criterion (R(2)(trial)) proposed by Buyse et al. are influenced by the magnitude of treatment effect with an application using real data. Methods: The criterion R(2)(trial) proposed by Buyse et al. (2000) is applied to the four data sets from colon cancer clinical trials (C-01, C-02, C-03 and C-04). Each clinical trial is analyzed separately for treatment effect on survival (true endpoint) and recurrence free survival (surrogate endpoint) and this analysis is done also for each center in each trial. Results are used for standard validation analysis. The centers were grouped by the Wald statistic in 3 equal groups. Results: Validation criteria R(2)(trial) were 0.641 95% CI (0.432-0.782), 0.223 95% CI (0.008-0.503), 0.761 95% CI (0.550-0.872) and 0.560 95% CI (0.404-0.687) for C-01, C-02, C-03 and C-04 respectively. The R(2)(trial) criteria changed by the Wald statistics observed for the centers used in the validation process. Higher the Wald statistic groups are higher the R(2)(trial) values observed. Conclusion: The recurrence free survival is not a good surrogate for overall survival in clinical trials with non significant treatment effects and moderate for significant treatment effects. This shows that the level of significance of treatment effect should be taken into account in validation process of surrogate endpoints.Yayın The effect of electrophysiological and neuroimaging findings on the prognosis of juvenile myoclonic epilepsy proband(Taylor and Francis Online, 2013) Aslan, Kezban; Bozdemir, Hacer; Yapar, Zeynep; Burgut, Hüseyin RefikBackground: Juvenile myoclonic epilepsy (JME) is a well-defined idiopathic generalized epileptic syndrome, and diagnostic criteria for JME are to have a normal brain imaging and clinical evidence of typical epileptic seizures. The aim of this study is to evaluate electrophysiological and neuroimaging findings of JME and determine their relationship with prognosis. Methods: Thirty-two patients (23 women and nine men) with a mean age of 22 (16–37) years were included in this study. Interictal electroencephalography (EEG), magnetic resonance imaging (MRI) and single photon emission computed tomography (SPECT) were carried out in all patients. Results: Analysis of premedication EEGs revealed primary generalized pattern activity in 75% (n524) and focal abnormalities in 18. 75% (n56). MRI was abnormal in seven (21. 88%) patients (two with arachnoid cyst, two with mild cerebral atrophy, two with ventricular enlargement and one with single gliotic lesion), and SPECT imaging detected hypoperfusion in 15 (47%) patients. Hypoperfusion was mostly found on the parietal lobe. Conclusion: We found that, after medication, only 6. 25% of EEGs had primary generalized pattern activity (p,0. 0001); nevertheless, the prognosis was good in patients who had typical EEG findings (p50. 106). The prognosis of patients with MRI abnormalities was grave (p50. 023). Twenty percent of the patients who had SPECT abnormalities were seizure free, and 80% of them had been partially controlled (p50. 059). There were no correlations between MRI abnormalities, EEG and SPECT findings.Yayın Empiric therapy in the management of febrile neutropenic cancer patients(Elsevier, 1993) Erkişi, Melek; Köksal, Fatih; Akan, Erol; Burgut, Hüseyin RefikThe effectiveness of empiric therapy was evaluated in 186 cancer patients who experienced 417 febrile and neutropenic episodes during the last 4 years. Ninety-eight patients experiencing 244 episodes were treated with empiric antibiotic therapy with mezlocillin and tobramycin. The response rate to the empiric treatment was 88% for bacterial infections and 58% for unexplained fever. Fluconazole 200 mg IV daily was added to the empiric antibiotic therapy in the subsequent 173 episodes experienced by 90 patients, of whom 12 had also been evaluated in the previous group. The addition of fluconazole improved the response rates to 90% in bacterial infections and to 86% in unexplained fever. In proven fungal infections in neutropenic patients, empiric antibiotic therapy combined with higher doses of fluconazole seemed beneficial. This study suggests that fluconazole 200 mg IV daily is an effective adjunct to empiric antibiotic therapy, but higher doses of fluconazole are preferable in proven fungal infections.Yayın Etiology of serum CA-125 in patients with endometriosis treated with a gonadotrophin-releasing hormone agonist (Buserelin)(Karger Publishing, 1994) Çetin, T; Vardar, Mehmet Ali; Demir, C.; Burgut, Hüseyin RefikFifty-six patients with pelvic endometriosis were treated with a buserelin dosage of 200 µg/day s.c. for 6 months. Scoring of the American Fertility Society (AFS) for endometriosis was performed by laparoscopy in all cases before and at the end of therapy. Serum CA-125 and estradiol levels were determined before the treatment, and monthly during therapy and follow-up (6 months; respectively). A slight positive correlation was found between serum CA-125 concentrations before treatment and AFS scores for adhesions only. Before treatment, serum CA-125 values also correlated slightly with total AFS scores of patients with adhesions. At the end of the 6-month therapy, no correlation was found between CA-125 concentrations and second-look AFS scores for implants and/or adhesions. Serum CA-125 and estradiol values were closely parallel to each other during and after therapy with the gonadotrophin-releasing hormone agonist buserelin. In conclusion, (1) adhesions may play a role in the elevation of serum CA-125 levels in endometriosis, and (2) a significant decrease in serum CA-125 values during the buserelin therapy may result from a rather different mechanism, such as ovarian suppression, other than the therapeutic effect of this agent.Yayın Evaluation of efficacy of immunotherapy in children with asthma monosensitized to Alternaria(Turkish National Pediatric Society, 2011) Kılıç, Mehmet; Altıntaş, Derya Ufuk; Yılmaz, Mustafa; Bingöl Karakoç, Gülbin; Burgut, Hüseyin Refik; Güneşer Kendirli, SevalSUMMARY: Kılıç M, Altıntaş DU, Yılmaz M, Bingöl-Karakoç G, Burgut R, Güneşer-Kendirli S. Evaluation of efficacy of immunotherapy in children with asthma monosensitized to Alternaria. Turk J Pediatr 2011; 53: 285-294. In this study, we aimed to evaluate the efficacy of specific immunotherapy (SIT) in children monosensitized to Alternaria. Sixteen children with bronchial asthma monosensitized to Alternaria were enrolled in the study. Patients were divided into two groups as the immunotherapy group (Group I; 9 patients) and control group (Group II; 7 patients). A significant reduction in bronchial responsiveness to methacholine and Alternaria was found in Group I after one year of SIT (p=0.03, p=0.006) in comparison to controls. Specific IgE levels were decreased in the immunotherapy group (p=0.001). Following allergen provocation, a rise in sputum eosinophil count was found to be lower in the SIT group compared to controls after one year (p=0.011), and sputum eosinophil cationic protein (ECP) levels did not change in the SIT group, while there was a statistically significant increase in controls. Our results demonstrated that SIT with Alternaria caused clear changes in airway responsiveness and serum-specific IgE levels. However, further longterm studies in large series should be carried out for clinical documentation of the efficacy of SIT in the treatment of children with Alternaria allergy.Yayın Evaluation of efficacy of immunotherapy in children with asthma monosensitized to Alternaria(Turkish National Pediatric Society, 2011) Kılıç, Mehmet; Altıntaş, Derya Ufuk; Yılmaz, Mustafa; Bingöl Karakoç, Gülbin; Burgut, Hüseyin Refik; Güneşer Kendirli, SevalIn this study, we aimed to evaluate the efficacy of specific immunotherapy (SIT) in children monosensitized to Alternaria. Sixteen children with bronchial asthma monosensitized to Alternaria were enrolled in the study. Patients were divided into two groups as the immunotherapy group (Group I; 9 patients) and control group (Group II; 7 patients). A significant reduction in bronchial responsiveness to methacholine and Alternaria was found in Group I after one year of SIT (p = 0.03, p = 0.006) in comparison to controls. Specific IgE levels were decreased in the immunotherapy group (p = 0.001). Following allergen provocation, a rise in sputum eosinophil count was found to be lower in the SIT group compared to controls after one year (p = 0.011), and sputum eosinophil cationic protein (ECP) levels did not change in the SIT group, while there was a statistically significant increase in controls. Our results demonstrated that SIT with Alternaria caused clear changes in airway responsiveness and serum-specific IgE levels. However, further long-term studies in large series should be carried out for clinical documentation of the efficacy of SIT in the treatment of children with Alternaria allergy.Yayın Evaluation of risk factors in patients with vulvovaginal candidiasis and the value of chromID Candida agar versus CHROMagar Candida for recovery and presumptive identifi cation of vaginal yeast species(Elsevier, 2011) Güzel, Ahmet Barış; Ilkit, Macit; Akar, Tuba; Burgut, Hüseyin Refik; Demir, Süleyman CansunVulvovaginal candidiasis (VVC), particularly the recurrent form, remains an intractable problem for clinicians, microbiologists, and patients. It is essential to confi rm the clinical diagnosis by mycological methods and avoid empirical therapy. The recovery of yeast in fungal culture, such as on Sabouraud dextrose agar, remains the gold standard for diagnosis. In this investigation, we examined 474 participants, including 122 (25.7%) with acute VVC cases, 249 (52.5%) who had recurrent VVC (RVVC) cases, and 103 (21.7%) healthy controls. We also administered a questionnaire to obtain information on patient lifestyle and medical, gynecological, and sexual history. In addition, we compared the performance of chromID Candida agar (CAN2) to CHROMagar Candida (CAC) and Sabouraud dextrose agar with gentamicin and chloramphenicol (SGC2). The yeasts were identifi ed by conventional methods including the germ tube test, microscopic morphology on cornmeal–Tween 80 agar, and the commercial API 20C AUX system. We detected yeasts in 60 of 122 (49.2%) patients with acute VVC cases, 110 of 249 (44.2%) with RVVC cases, and in 35 of 103 (34%) healthy controls ( P 0.07). A total of 205 samples were found to be positive for fungi (43.2%), of which 176 (85.9%) were monofungal, and 29 (14.1%) were polyfungal. In addition, 198 of these samples (96.6%) were positive on CAN2, 195 (95.1%) on CAC, 189 (92.2%) on SGC2, and 183 (89.3%) samples on all three ( P 0.17). The 234 yeast isolates recovered were C. albicans ( n 118), C. glabrata ( n 82), C. kefyr ( n 11), C. krusei ( n 9), C. lipolytica ( n 3), C. colliculosa ( n 2), C. parapsilosis ( n 2), C. pelliculosa ( n 2), C. tropicalis ( n 2), and other species of Candida ( n 3). Of the 29 polyfungal populations, 28 (96.6%) were detected in CAN2, 25 in (86.2%) CAC, and 25 (86.2%) on both ( P 0.35). Notably, we detected the high predominance of C. albicansC. glabrata (86.2%) in polyfungal populations. Briefl y, the detection of C. albicans after 24 h of incubation was easier on CAN2 (64.4%) than on CAC (25.4%). This study showed that CAN2 is a rapid and reliable medium for immediate identifi cation of C. albicans and for detecting polyfungal populations in vaginal specimens. We observed that the use of antibiotics, intrauterine devices, as well as, perineal laceration, short anovaginal distance ( 3 cm), and genital epilation in common areas are predisposing factors for RVVC ( P 0.001). In addition, we detected that the use of menstrual pad, using an (IUD), and having a history of childbirth increased the risk of both acute and recurrent VVC ( P 0.01), whereas the use of a daily pad and walking daily signifi cantly decreased the risk of both acute and recurrent VVC ( P 0.01).Yayın An evaluation of risk factors in pregnant women with candida vaginitis and the diagnostic value of simultaneous vaginal and rectal sampling(Springerlink, 2011) Güzel, Ahmet Barış; Ilkit, Macit; Burgut, Hüseyin Refik; Ürünsak, İbrahim Ferhat; Özgünen, Fatma TuncayIn this study, we investigated the epidemiological characteristics of VVC among pregnant women. We conducted a prospective survey among 372 pregnant women to investigate the prevalence, clinical forms, etiological agents, and predisposing factors of VVC. In addition, we determined the relationship between vaginal and rectal flora by simultaneously obtaining one high vaginal swab and one rectal swab from each patient using sterile cottontipped swabs. Furthermore, we compared the recovery and identification performances of chromID Candida agar to Sabouraud dextrose agar with gentamicin and chloramphenicol. Clinically and mycologically confirmed cases of VVC were detected in 139 (37.4%) and vaginal colonization described in 42 (11.3%) of 372 pregnant women. Rectal cultures were also positive in 98 of the 139 (70.5%) VVC cases. Candida albicans and C. glabrata were identified in vaginal samples in 58.0 versus 19.0% and from rectal samples in 49.0 versus 13.5%, respectively. Increases in gestational week and gravidae were identified to be statistically significant in patients with acute VVC (AVVC) and symptomatic recurrent VVC (RVVC), and asymptomatic RVVC (P = 0.04 and P = 0.03, respectively). In the laboratory diagnosis of VVC, specifically tailored chromogenic media are reliable tools for both the recovery and rapid identification of common Candida spp., particularly C. albicans, as well as for the detection of polyfungal populations in vaginal samples (P[0.05). In addition, rectal colonization is a common finding in cases of AVVC and symptomaticRVVC cases and corresponds well with the presence of the same yeast species in the vagina.Yayın Evaluation of score for neonatal acute physiology and perinatal extension II and clinical risk index for babies with additional parameters(Wiley Online Library, 2016) Asker, Hüseyin Selim; Satar, Mehmet; Yapıcıoğlu Yıldıztaş, Hacer; Mutlu, Birgül; Mutlu Özyurt, Banu; İpek, Mehmet Şah; Sivaslı, Ercan; Taviloğlu, Şafak; Çelik, Yalçın; Özcan, Kenan; Burgut, Hüseyin Refik; İlker, ÜnalBackground: The aim of this study was to determine mortality risk by calculating Score for Neonatal Acute Physiology and Perinatal Extension II (SNAP-PE-II) and Clinical Risk Index for Babies (CRIB) score, and evaluate prediction of the effects of antenatal corticosteroid and surfactant treatment on mortality. Methods: This multicenter study was conducted simultaneously in five different centers in four different provinces in Southern Turkey between July 2012 and July 2013. A total of 1668 inborn subjects hospitalized in the neonatal intensive care unit within the first 12 h of delivery, and meeting the selection criteria, were included in the study, and CRIB and SNAP-PE-II were used to determine mortality. Results: The SNAP-PE-II scoring system was applied to all patients, and the CRIB scoring system was used for 310 newborns with gestational age <32 weeks and weighing <1500 g. Of the 1668 patients, 188 died (mortality rate, 11.3%). Cut-off was found to vary with center, which changed specificity and sensitivity of the mortality scores. SNAP-PE-II significantly predicted mortality (P < 0.05) compared with CRIB. SNAP-PE-II also successfully predicted mortality in the group receiving antenatal corticosteroid compared with the group not receiving antenatal corticosteroid. Conclusion: SNAP-PE-II was a significant predictor of mortality in newborns with birthweight <1500 g compared with CRIB, and assessment of antenatal corticosteroid use in conjunction with SNAP-PE-II increased the accuracy of the prediction of mortality.Yayın Expression of fibroblast growth factor receptor 1, fibroblast growth factor 2, phosphatidyl inositol 3 phosphate kinase and their clinical and prognostic significance in early and advanced stage of squamous cell carcinoma of the lung(Wiley Online Library, 2015) Usul Afşar, Çiğdem; Şahin, Berksoy; Gunaldi, Meral; Kılıç Bağır, Emine; Gumurdulu, Derya; Burgut, Hüseyin Refik; Erkişi, Melek; Kara, İsmail Oğuz; Paydaş, Semra; Karaca, Feryal; Erçolak, VehbiAim: Non-small cell lung carcinoma is the leading cause of cancer related to death in the world. Squamous cell lung carcinoma (SqCLC) is the second most frequent histological subtype of lung carcinomas. Recently, growth factors, growth factor receptors, and signal transduction system-related gene amplifications and mutations are extensively under investigation to estimate the prognosis and to develop individualized therapies in SqCLC. In this study, besides the signal transduction molecule phosphatidyl inositol-3-phosphate kinase (IP3K) p110?, we explored the expressions of fibroblast growth factor 2 (FGF2) and receptor-1 (FGFR1) in tumor tissue and also their clinical and prognostic significance in patients with early/advanced SqCLC. Materials and methods: From 2005 to 2013, 129 patients (23 early, 106 advanced disease) with a histopathological SqCLC diagnosis were selected from the hospital files of Cukurova University Medical Faculty for this study. Two independent pathologists evaluated FGFR1, FGF2, and PI3K (p110?) expressions in both tumor and stromal tissues from 99 of the patients with sufficient tissue samples, using immunohistochemistry. Considering survival analysis separately for patients with both early and advanced stage diseases, the relationship between the clinical features of the patients and expressions were evaluated by univariate and multivariate analyses. Results: FGFR1 expression was found to be low in 59 (60%) patients and high in 40 (40%) patients. For FGF2; 12 (12%) patients had high, 87 (88%) patients had low expression and for IP3K; 31 (32%) patients had high and 66 (68%) patients had low expressions. In univariate analysis, overall survival (OS) was significantly associated with stage of the disease and the performance status of the patient (P<0.0001 and P<0.001). There was no significant difference in OS of the patients with either low or high expressions of FGFR1, FGF2, and IP3K. When the patients with early or advanced stage disease were separately taken into consideration, the relationship did not differ, either. Any of FGFR1, FGF2 or IP3K expressions was not found predictive for the treatment of early or advanced staged patients. On the other hand, the expressions of both FGFR1 and FGF2 were significantly different with respect to smoking, scar of tuberculosis and scar of radiotherapy (P=0.002; P=0.06 and P=0.05, respectively). Discussion: There has not been identified an effective individualized treatment for SqCLC yet. Therefore, in order to be able to develop such a treatment in the future, it is essential to identify the genetic abnormalities that are responsible for the biological behaviors and carcinogenesis of SqCLC. Although we could not show the prognostic and predictive significance of FGFR1, FGF2 and IP3K expressions in SqCLC, we determined the expression rates of FGFR1, FGF2 and IP3K as a reference for Turkish patients. In conclusion, we want to put some emphasis on the fact that, pulmonary fibrosis which is a late complication of radiotherapy at stage III disease, and the scar of tuberculosis could be associated with FGFR1 and FGF2 expressions.Yayın Human papillomavirus detection in cervical smears and cervical tissue excised by the Loop Electrosurgical Excision Procedure (LEEP). Diagnostic value of cytology, colposcopy and histology(National Center for Biotechnology Information, 1995) Vardar, Mehmet Ali; Altıntaş, Hilal; Doran, F.; Arıdoğan, Nihat; Demir, C.; Burgut, Hüseyin Refik; Varınlı, S.A study was performed on 176 patients with benign cervical lesions. Human papillomavirus (HPV) structural antigens which were stained with the immunoperoxidase staining were sought for in cervical smears and in cervical tissues excised by the loop electrosurgical excision procedure (LEEP). HPV infection was found in 16.4% of the patients. Furthermore, cytologic screening on Papanicolaou (PAP) smears, colposcopy, and histologic examinations of whole transformation zones excised by the LEEP were performed. The diagnosis of HPV was based on the positive immunoperoxidase staining of either the exfoliated cells or the cervical tissue. HPV was detected on 5.6% of the PAP smears. The reliability of cytologic colposcopic, and histologic diagnosis of HPV was investigated.
