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    Structural characterization and cell adhesion properties to lipps patterned ti implants
    (Maltepe Üniversitesi, 2019) Dudko, J.; Deineka, V.; Liubchak, I.
    Introduction: Geometry and topography of the implant surface are critical for the shortand long-term success of implantation. Quality of osteointegration related to their surface properties. Various treatments have been developed to modify the implants surface, include machining/micromachining, sandblasting, etc. Last decades numerous studies sown advantages of nanostructures for cell attachment. The aim of research was structural characterization and cell proliferation on lasermodified titanium implants Materials and Methods: In current research the surface of Titanium samples (polished and sandblasted) was patterned with Laser Induced Periodic Surface Structures in order to provide high regular nanostructure. 10 mm in diameter were treated generating LIPSS under two different parameters sets – LIPSS-1 and LIPSS-2. The surface morphology and chemistry were investigated both by secondary electrons imaging and EDS analysis. Contact angle was measured using video-based optical contact angle measuring instrument (OCA 15 EC, Data Physics, USA). Human primary osteoblasts were used for cell adhesion and proliferation assessment 1, 3 and 7 days. Results: LIPSS provide formation of homogeneous and regular patters both on polished and sandblasted surfaces with size ranges between 400 and 600 nm (fig. 1). There was no difference in patterns size between LIPSS-1 and LIPSS-2 regimens. CA after LIPSS increase in 23% (mode 1) and 35% (mode 2) compare the non-treated ones. Cell culture proved better cell adhesion for LIPSS modified surfaces with excellent proliferation rate compare the non-treated ones. LIPSS-2 provided better adhesion properties. Discussion: LIPSS modified polished and sandblasted surface have grate potential for biomedical application due to developed structure and cell adhesion properties. Acknowledgment: Authors thanks to prof. Leonardo Orazi (University Reggio Emilia, Italy) for LIPSS patterning. This research supported by H2020 Marie Sk?odowska-Curie Actions, grant NanoSurf 777926