The use of mesenchymal stem cell therapy in the treatment of cerebrovascular accident
Küçük Resim Yok
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Maltepe Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: Stroke is a neurological disorder characterized by a blockage of blood vessels. Annually in Kazakhstan 40 thousand people suffer a stroke, 5 thousand of them die in the first 10 days of illness. The main method of treating stroke in Kazakhstan is thrombolytic therapy, for hemorrhagic strokes decompressive craniotomy is prescribed. Purpose of the research. Approbation of an experimental model of ischemic stroke for testing biologically active material and determination of neuron-specific enolase and S-100 protein as a cerebrovascular accident (CVA) factor. Methods: The experiments were carried out on male rats weighing 240-318 g. To simulate ischemic stroke was chosen the occlusion method, which is characterized by the highest probability of CVA in the brain. The occlusion of the common carotid artery (CCA) was performed near its bifurcation by ligation of the vessel. The rats were anesthetized with ketamine (125 mg/kg). The ligatures were kept on the vessels for 20 minutes. Mesenchymal stem cells (2 x 10^6/ml) were administered to rats of the experimental group immediately after the operation, one day after the operation, and three days after the operation. Venous blood samples for measurement of neuron-specific enolase (NSE) and S-100 concentrations were taken at four time points: before the operation (basal sample), immediately after CVA, 60 minutes after CVA, and after 3 days. Condition assessment. The neurological status was assessed using the McGrow method of assessing neurological deficits. The open field technique was used to assess the exploratory behavior. Decapitation of intact group rats was carried out 1 and 3 days after CVA. Results: The highest stroke index was observed immediately after the operation and consisted of such symptoms as unilateral ptosis, paralysis and paresis of the limbs, coma, circus movements, poor coordination of movements, lethargy and tremor. After the administration of MSCs, concentrations of NSE, S-100 decreased and were close to the results of the analyzes of the intact group. Necropsy of rats with injected MSCs showed that the volume of hemorrhages was less than in rats with stroke without treatment.
Açıklama
Anahtar Kelimeler
Kaynak
International Faculty of Medical Sciences and Nursing(MEDNS) Student Congress
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Baibussunova, A. (2021). The use of mesenchymal stem cell therapy in the treatment of cerebrovascular accident. International Faculty of Medical Sciences and Nursing(MEDNS) Student Congress, Maltepe Üniversitesi. s. 84-85.
