Pronuclear synchronization and nuclear morphology of mature and in vitro matured oocytes in the rat: an ultrastructural study

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dc.authorid0000-0002-0717-1756en_US
dc.contributor.authorBaykal, B.
dc.contributor.authorZeteroğlu, S.
dc.contributor.authorOnalan, G.
dc.contributor.authorCeyhan, S. T.
dc.contributor.authorErgur, R.
dc.date.accessioned2024-07-12T21:04:06Z
dc.date.available2024-07-12T21:04:06Z
dc.date.issued2005en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractThe objective of this study was to evaluate synchronous and asynchronous pronucleus (PN) formation and the related patterns of juxtapositional nucleolus (n) formation in immature (prophase I [PI] and metaphase I [MI]) and mature (metaphase II [MII]) oocytes after fertilization, both ultrastructurally and at the level of light microscope. A single dose of 15 IU gonadotrophin was injected subcutaneously to twenty four 26-wk-old, female Wistar rats to induce ovulation. Human chorionic gonadotrophin (4 IU) was administered 40 h later, and after 4–6 h the ovaries were dissected, and the oocytes were aspirated. A total of 214 rat oocytes were classified according to a maturation index as follows: group I, 80 PI oocytes; group II, 50 MI oocytes; and group III, 84 MII oocytes. Immature oocytes were in vitro matured for 18–36 h. Spermatozoa were acquired by microepididymal sperm aspiration and processed using swim-up technique. Intracytoplasmic sperm injection was performed on mature oocytes after 2 h of incubation and on in vitro matured (IVM) oocytes 4 h after maturation. Pronuclear synchronization [both pronucleases (PNs) centrally located, equal sized, with equal numbers and sizes of juxtapositional nucleoli (Nn)] was observed in fertilized oocytes. Asynchronous PN formation (diversity between male and female PNs, related to dimensions, localization, and the number of Nn) in groups I, II, and III was found in 75, 86, and 47% of preembryos, respectively. There was a significant difference of synchronous pronuclear formation between mature and IVM oocytes (P<0.05). In IVM oocytes, asynchronous PN formation is high, and juxtapositional pronucleolar patterns are observed to be low by transmission electron microscope (TEM).en_US
dc.identifier.citationCincik, M., Baykal, B., Zeteroglu, S., Onalan, G., Ceyhan, S.T. ve Ergur, R. (2005) Pronuclear synchronization and nuclear morphology of mature and in vitro matured oocytes in the rat: An ultrastructural study. In Vitro Cell.Dev.Biol.-Animal. 41(8), s. 272–277.en_US
dc.identifier.endpage277en_US
dc.identifier.issn1071-2690
dc.identifier.issue8en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage272en_US
dc.identifier.urihttps://link.springer.com/article/10.1290/0505030R.1#citeas
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3753
dc.identifier.volume41en_US
dc.institutionauthorCıncık, Mehmet
dc.language.isoenen_US
dc.publisherSpringerlinken_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology - Animalen_US
dc.relation.isversionof10.1290/0505030R.1en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.snmzKY01227
dc.subjectpronucleusen_US
dc.subjectasynchronous pronuclear morphologyen_US
dc.subjectnucleolusen_US
dc.subjectTEMen_US
dc.titlePronuclear synchronization and nuclear morphology of mature and in vitro matured oocytes in the rat: an ultrastructural studyen_US
dc.typeArticle
dspace.entity.typePublication

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