Protective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileum

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dc.authorid0000-0003-2651-8353en_US
dc.authorid0000-0001-9328-9373en_US
dc.authorid0000-0002-4474-7371en_US
dc.contributor.authorÖzaçmak, Veysel Haktan
dc.contributor.authorArslan, Seyfullah Oktay
dc.contributor.authorAltaner, Şemsi
dc.date.accessioned2024-07-12T21:06:53Z
dc.date.available2024-07-12T21:06:53Z
dc.date.issued2005en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractFree radicals derived from molecular oxygen have been reported to be responsible for changes in motility and mucosal damage observed in intestinal ischemia-reperfusion injury. Melatonin has been considered as an antioxidant that prevents injuries resulted from I/R in various tissues. The present study was designed to determine the effect of melatonin on the contractile responses of acetylcholine (Ach) and KCl, on malondialdehyde (MDA), a product of lipid peroxidation, and reduced glutathione (GSH) levels and to assess histopathological changes in the smooth muscle of terminal ileum subjected to ischemia-reperfusion. The intestinal ischemia-reperfusion was induced by occlusion of superior mesenteric artery of rat for 30 min, followed by a period of reperfusion for 3 h. Melatonin at doses of 10 or 50 mg/kg was administered via the tail vein in 5 min prior to reperfusion. Following reperfusion, segments of terminal ileum were rapidly taken and transferred into isolated organ bath and responses to Ach and KCl were recorded. Samples of terminal ileum were also taken for measuring the MDA and GSH levels. EC50 values of these contracting substances were seriously reduced in the ischemia-reperfusion group compared to that of the sham-operated control group. The decreased contraction response to Ach and KCl was significantly ameliorated by a dosage of 50 mg/kg of melatonin, while not by a dosage of 10 mg/kg. Similar pattern of the effect was observed in the tissue levels of MDA and GSH as well as in histological improvement. Melatonin appeared to be restoring the amounts of tissue MDA and GSH back to about control levels. These results suggest that the high dose of melatonin not only physiologically but also biochemically and morphologically could be useful to normalize contractility injured by oxidative stress in intestinal ischemia-reperfusion.en_US
dc.identifier.citationÖzaçmka, V.H., Arslan, S.O., Altaner, Ş. ve Aktaş, R. G. (2005). Protective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileum. Life Sicences, Elsevier. 76(14), s. 1575-1588.en_US
dc.identifier.endpage1588en_US
dc.identifier.issn0024-3205
dc.identifier.issue14en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1575en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S0024320504010124?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3894
dc.identifier.volume76en_US
dc.institutionauthorAktas, Ranan Gulhan
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofLife Sciencesen_US
dc.relation.isversionof10.1016/j.lfs.2004.08.031en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.snmzKY02197
dc.subjectContractile responseen_US
dc.subjectIleumen_US
dc.subjectIschemia-reperfusionen_US
dc.subjectMelatoninen_US
dc.subjectRaten_US
dc.titleProtective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileumen_US
dc.typeArticle
dspace.entity.typePublication

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