Quantitative Analysis of Immunogold Labeling for Basic Fibroblast Growth Factor According to the Activational Stages of Mast Cells

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dc.authorid0000-0001-9452-5251en_US
dc.contributor.authorAktas, Ranan Gulhan
dc.contributor.authorKayton, Robert J.
dc.contributor.authorCaglar, Mine
dc.contributor.authorOktayer, Adviye Gozde
dc.date.accessioned2024-07-12T21:44:51Z
dc.date.available2024-07-12T21:44:51Z
dc.date.issued2013en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractOBJECTIVE: To analyze whether immunogold labeling density for basic fibroblastic growth factor in granules is compatible with the activation stage of mast cells. STUDY DESIGN: Cytoplasmic features and granules of 46 mast cells were examined at the ultrastructural level. The cells were classified according to their activation stage, namely, whether resting, initially activated, fully degranulated or piecemeal degranulated. Granules were classified as electron lucent, moderate or dense granules. Gold particles per secretory granules in the cells were counted. Recently described quantitative analysis techniques were used for evaluation. RESULTS: There is a statistically meaningful relationship between the activation stage of mast cells and their immunogold labeling density. The number of different types of granules encountered in a cell depends on the type of the cell. The distribution of gold particles among the secretory granules depends upon the cell. The type of granule does not have an individual effect on the number of particles, as indicated by an overall statistical analysis of granules, cells and their interaction effects. CONCLUSION: A count of gold particles in the cells can be used as a strong biological indicator. Therefore the number of gold particles might be very useful for comparative studies related to the secretion of this growth factor under different conditions.en_US
dc.identifier.endpage315en_US
dc.identifier.issn0884-6812
dc.identifier.issue6en_US
dc.identifier.pmid24617036en_US
dc.identifier.startpage306en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/7761
dc.identifier.volume35en_US
dc.identifier.wosWOS:000341746300002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSCI PRINTERS & PUBL INCen_US
dc.relation.ispartofANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY00216
dc.subjectactivation stageen_US
dc.subjectbasic fibroblast growth factoren_US
dc.subjectbasic fibroblast growth factor receptor 1, humanen_US
dc.subjectBFGFR protein, humanen_US
dc.subjectimmunohistochemistryen_US
dc.subjectimmunogold labelingen_US
dc.subjectimmunogold techniquesen_US
dc.subjectimmunolabeling techniquesen_US
dc.subjectmast cellsen_US
dc.titleQuantitative Analysis of Immunogold Labeling for Basic Fibroblast Growth Factor According to the Activational Stages of Mast Cellsen_US
dc.typeArticle
dspace.entity.typePublication

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