The effects of temperature and carbondioxide changes related to type i collagen presence on hepatocellular carcinoma cells
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Marcau Flavius Cristian
Erişim Hakkı
Attribution-NonCommercial-NoDerivs 3.0 United States
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
The early prognosis of hepatocellular carcinoma (hcc) is extremely difficult and treatment options are still very limited. The study aims to examine the effects of temperature and carbondioxide changes related to the presence of type i collagen, one of the most common components of the extracellular matrix. Twodimensional hepg2 cell cultures were maintained in both classical culture conditions and type i collagen-coated culture vessels at +4c°, +24c°, and +37c°. A comparative examination of phenotypic changes was performed after 24, 48, 72, 96, and 120 hours. The cells that are in the incubators at 37c° with 5% co2 demonstrated normal growth pattern. Albumin expression was higher in the cells on type i collagen coated surfaces. Carbondioxide depletion caused morphological changes and a gradual decrease in viability. The cancer cells on type i collagencoated surfaces presented significant phenotypic changes in all groups. The results of the study suggest that the cells on type i collagen-coated surfaces were more resistant to the temperature and the carbondioxide changes in the microenvironment. Further studies related to the mechanism(s) that leads to this rezistance in cancer cells might be insightful for the development of new therapeutics.
Açıklama
Anahtar Kelimeler
Cancer, extracellular matrix, hepg2, collagen type i
Kaynak
Research and Science Today
WoS Q Değeri
Scopus Q Değeri
Cilt
2
Sayı
20
Künye
Akbulut, Z., Kavaklı, A. Ö., Karagöz Kavaklı, A., Maras, H. ve Gülhan Aktaş, R. (2020). The effects of temperature and carbondioxide changes related to type i collagen presence on hepatocellular carcinoma cells. Research and Science Today, Marcau Flavius Cristian. 2(20), s. 193-204.
