Malign melanom olgularında intratümöral mast hücre miktarı ile histopatolojik prognostik parametrelerin karşılaştırılması
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Maltepe Üniversitesi
Erişim Hakkı
CC0 1.0 Universal
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Amaç: Malign melanom (MM), insidansı giderek artan, melanositlerden köken alan bir neoplazidir. Mast hücrelerinin alerjik olaylardaki rolü iyi bilinmekte ancak solid tümörlerde mikroçevre ve prognoz ile ilişkileri kesinlik kazanmamıştır. MM’da epidermal değişikliklerin önemli bir prognostik parametre olan ülser gelişimi ve tümör hücre büyümesi ile ilişkili olabileceği düşünülmektedir. Bu çalışmada prognostik önemi kanıtlanmış parametreler ile mast hücreleri ve epidermal değişiklikler arasındaki ilişkiyi göstermek amaçlandı. Materyal ve Metodlar: Çalışmamıza Ocak 2015-Şubat 2019 tarihleri arasında eksizyonel biyopsi ile MM tanısı alan 40 olgu dahil edilmiştir. Olguların H&E boyalı preparatlarından epidermal değişiklikler (bazoskuamoz dejenerasyon varlığı, incelme, rete oluşumu, epidermis-dermis arası kleft oluşumu), plazma hücre sayısı ve ek olarak yapılan Giemsa histokimyasal boyası ile intratümöral mast hücre sayısı değerlendirilmiştir. Bu bulgular prognostik parametreler ile karşılaştırılmıştır. Bulgular: Olguların %32’si (n=13) kadın, %67.5’u (n=27) erkekti. Bazoskuamoz dejenerasyon varlığına göre Clark seviyesi, ülserasyon, lenfositik infiltrasyon arasında anlamlı farklılık saptanmadı. Mast hücre sayısı ile Breslow kalınlığı, ülserasyon, lenfosit infiltrasyonu, mitoz sayısı ile anlamlı ilişki saptanmadı. Plazma hücre varlığına göre prognostik faktörler ile anlamlı farklılık saptanmış olup zeminde nevüs görülen olgularda plazma hücreleri sayıca daha düşüktür. Sonuç: Olguların çoğunda epidermal değişikliklerin gözlenmesi, bu bulgunun malign melanomun histopatolojik karakteristik bulgularından biri olabileceğini düşündürmektedir. Bazı çalışmalarda epidermal değişikliklerin ülser gelişiminin öncüsü olabileceği öne sürülmektedir, bu da prognostik öneminin olabileceğini gösterir. Çalışmamızda intratümöral mast hücreleri ile prognostik parametreler arasında istatistiksel olarak anlamlı ilişki görülmedi. Mast hücrelerinin hem pro-tümor hem anti-tümör etki gösteriyor olabileceği düşünülmektedir
Aim: Malignant Melanoma (MM) is a neoplasia originates from melanocytes. The incidence of MM has been increasing. The role of mast cells in allergic reactions is well known but their relationship with microenvironment and prognosis in solid tumors is uncertain. It is thought that epidermal changes in MM may be related to ulcer development and tumor cell growth which are important prognostic factors. The aim of the study is to show the relationship between proven parameters, mast cells and epidermal changes. Materials and Methods: Forty patients diagnosed with MM by excisional biopsy between January 2015-February 2019 were included in the study. Plasma cell count and epidermal changes (presence of baso-squamous degeneration, thinning, formation of rete, kleft formation between of epidermis and dermis) were evaluated from H&E stained specimens. The intratumoral mast cell counts was evaluated with Giemsa. These findings were compared with prognostic factors. Results: MM cases consisted of 32% (n=13) female and 67% (n=27) male. There was no significant difference between Clark level, ulceration and lymphocytic infiltration according to presence of basosquamous degeneration. There was no significant relation between mast cells count and Breslow thickness, ulceration, lymphocyte infiltration and mitosis count. There was a significant difference with prognostic factors according to the presence of plasma cells. According to the presence of plasma cells, there was a significant difference with prognostic factors. Plasma cell number was lower in the cases with nevus at the beginning of the tumor. Conclusion: The observation of epidermal changes in the majority of cases suggests that this finding may be one of the histopathological characteristic findings of malignant melanoma. Some studies suggest that epidermal changes may be the precursor to ulcer development, suggesting that it may have prognostic significance. In our study, there was no statistically significant relationship between intratumoral mast cells and prognostic parameters. Mast cells are thought to have both pro-tumor and anti-tumor effects.
Aim: Malignant Melanoma (MM) is a neoplasia originates from melanocytes. The incidence of MM has been increasing. The role of mast cells in allergic reactions is well known but their relationship with microenvironment and prognosis in solid tumors is uncertain. It is thought that epidermal changes in MM may be related to ulcer development and tumor cell growth which are important prognostic factors. The aim of the study is to show the relationship between proven parameters, mast cells and epidermal changes. Materials and Methods: Forty patients diagnosed with MM by excisional biopsy between January 2015-February 2019 were included in the study. Plasma cell count and epidermal changes (presence of baso-squamous degeneration, thinning, formation of rete, kleft formation between of epidermis and dermis) were evaluated from H&E stained specimens. The intratumoral mast cell counts was evaluated with Giemsa. These findings were compared with prognostic factors. Results: MM cases consisted of 32% (n=13) female and 67% (n=27) male. There was no significant difference between Clark level, ulceration and lymphocytic infiltration according to presence of basosquamous degeneration. There was no significant relation between mast cells count and Breslow thickness, ulceration, lymphocyte infiltration and mitosis count. There was a significant difference with prognostic factors according to the presence of plasma cells. According to the presence of plasma cells, there was a significant difference with prognostic factors. Plasma cell number was lower in the cases with nevus at the beginning of the tumor. Conclusion: The observation of epidermal changes in the majority of cases suggests that this finding may be one of the histopathological characteristic findings of malignant melanoma. Some studies suggest that epidermal changes may be the precursor to ulcer development, suggesting that it may have prognostic significance. In our study, there was no statistically significant relationship between intratumoral mast cells and prognostic parameters. Mast cells are thought to have both pro-tumor and anti-tumor effects.
Açıklama
Anahtar Kelimeler
Epidermal değişiklikler, Malign melanom, Mast hücreler, Epidermal changes, Malignant melanoma, Mast cells
Kaynak
Maltepe Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
12
Sayı
1
Künye
Yalçın, Ö. ve Erdem, Z. B. (2020). Malign melanom olgularında intratümöral mast hücre miktarı ile histopatolojik prognostik parametrelerin karşılaştırılması / Comparison of prognostic parameters, intratumoral mast cells and epidermal changes in malignant melanoma patients. Maltepe Tıp Dergisi, 12(1), s.11-15.
