Effect of solution and post-mortem time on mechanical and histological properties of liver during cold preservation

Basit Öğe Kaydı
dc.authorid0000-0003-3411-6215en_US
dc.authorid0000-0002-4474-7371en_US
dc.authorid0000-0002-6382-7334en_US
dc.contributor.authorAyyildiz, Mehmet
dc.contributor.authorGulhan Aktas, Ranan
dc.contributor.authorBasdogan, Cagatay
dc.date.accessioned2024-07-12T21:02:08Z
dc.date.available2024-07-12T21:02:08Z
dc.date.issued2014en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractBACKGROUND: In liver transplantation, the donor and recipient are in different locations most of the time, and longer preservation periods are inevitable. Hence, the choice of the preservation solution and the duration of the preservation period are critical for the success of the transplant surgery. OBJECTIVE: In this study, we examine the mechanical and histological properties of the bovine liver tissue stored in Lactated Ringer's (control), HTK and UW solutions as a function of preservation period. METHODS: The mechanical experiments are conducted with a shear rheometer on cylindrical tissue samples extracted from 3 bovine livers and the change in viscoelastic material properties of the bovine liver is characterized using the fractional derivative Kelvin–Voigt Model. Also, the histological examinations are performed on the same liver samples under a light microscope. RESULTS: The results show that the preservation solution and period have a significant effect on the mechanical and histological properties of the liver tissue. The storage and loss shear moduli, the number of the apoptotic cells, the collagen accumulation, and the sinusoidal dilatation increase, and the glycogen deposition decreases as the preservation period is longer. CONCLUSIONS: Based on the statistical analyses, we observe that the liver tissue is preserved well in all three solutions for up to 11 h. After then, UW solution provides a better preservation up to 29 h. However, for preservation periods longer than 29 h, HTK is a more effective preservation solution based on the least amount of change in mechanical properties. On the other hand, the highest correlation between the mechanical and histological properties is observed for the liver samples preserved in UW solution.en_US
dc.identifier.citationAyyılıdz, M., Gulhan Aktas, R. ve Basdogan, C. (2014). Effect of solution and post-mortem time on mechanical and histological properties of liver during cold preservation. Biorheology, IOS Press. 51(1), s. 47-70.en_US
dc.identifier.endpage70en_US
dc.identifier.issn1878-5034
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage47en_US
dc.identifier.urihttps://content.iospress.com/articles/biorheology/bir14007
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3584
dc.identifier.volume51en_US
dc.language.isoenen_US
dc.publisherIOS Pressen_US
dc.relation.ispartofBiorheologyen_US
dc.relation.isversionof10.3233/BIR-14007en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.snmzKY00428
dc.subjectLiveren_US
dc.subjectrheologyen_US
dc.subjecthistologyen_US
dc.subjectmechanical characterizationen_US
dc.subjectpreservationen_US
dc.subjecttransplantationen_US
dc.titleEffect of solution and post-mortem time on mechanical and histological properties of liver during cold preservationen_US
dc.typeArticle
dspace.entity.typePublication

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