Micro RNA-126 coordinates cell behavior and signaling cascades according to characteristics of breast cancer cells

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dc.authorid0000-0003-3771-3277en_US
dc.contributor.authorTurgut Coşan, Didem
dc.contributor.authorÖner, Çağrı
dc.contributor.authorŞahin, F. Mutlu
dc.date.accessioned2024-07-12T21:02:57Z
dc.date.available2024-07-12T21:02:57Z
dc.date.issued2016en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractBACKGROUND: Micro RNA-126 is known to enhance apoptotic processes and also plays a role in vascular growth through the regulation of vascular endothelial growth factor-mediated signaling, angiogenesis, and vascular integrity. OBJECTIVES: We aimed to determine the role of miR-126 in breast cancer cell lines with a variety of different characteristics to evaluate its interaction with certain cancer-related molecules and mechanisms. METHODS: To determine the effect of presence and absence of miR-126 in MCF-7 and MDA-MB-231 breast cancer cells, miR-126 mimics and inhibitor were transfected. miRNA and gene expressions were observed by using RT-PCR. Viability, proliferation, adhesion, invasion and lateral motility assays were performed to determine cell behavior changes. RESULTS: miR-126 is more effective on MDA-MB-231 cells on cell behavior. We observed an increase in miR-126 expression when miR-126 mimics was transfected to MCF-7 and MDA-MB-231 cells. Also, there was a decrease in miR-126 expression when MCF-7 and MDA-MB-231 cells were transfected with miR-126 inhibitor. Furthermore, presence and absence of miR-126 modulated the gene expressions of VEGF/PI3K/AKT and MAPK signaling in MCF-7 and MDA-MB-231. CONCLUSION: Our study showed that miR-126 is in a state of interaction with a multitude molecules playing a role in breast cancer. According to obtained data, we can say that miR-126 may be more effective in inhibition of metastatic breast cancer (Tab. 4, Fig. 3, Ref. 46).en_US
dc.identifier.citationTurgut Coşan, D., Öner, Ç. ve Şahin, F. M. (2016). Micro RNA-126 coordinates cell behavior and signaling cascades according to characteristics of breast cancer cells. Bratislava Medical Journal, AEPress. 117(11), s. 639-647.en_US
dc.identifier.endpage647en_US
dc.identifier.issn1336-0345
dc.identifier.issue11en_US
dc.identifier.startpage639en_US
dc.identifier.urihttp://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=4995&category_id=129&option=com_virtuemart&vmcchk=1&Itemid=1
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3594
dc.identifier.volume117en_US
dc.institutionauthorÖner, Çağrı
dc.language.isoenen_US
dc.publisherAEPressen_US
dc.relation.ispartofBratislava Medical Journalen_US
dc.relation.isversionof10.4149/BLL_2016_124en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00450
dc.subjectbreast canceren_US
dc.subjectEgfl-7en_US
dc.subjectmetastasisen_US
dc.subjectmicro RNA-126en_US
dc.subjectmotilityen_US
dc.subjectsignaling pathwaysen_US
dc.titleMicro RNA-126 coordinates cell behavior and signaling cascades according to characteristics of breast cancer cellsen_US
dc.typeArticle
dspace.entity.typePublication

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