Time-lapse (tam zamanli görüntüleme sistemi) ile takip edilen IVF sikluslarinda, teratozoospermi ile normozoospermi vakalarinin embriyo morfokinetiğinin retrospektif karşılaştırılması
Küçük Resim Yok
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Maltepe Üniversitesi
Erişim Hakkı
CC0 1.0 Universal
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Amaç: Çalışmanın amacı, ICSI sikluslarında sperm morfolojisi ile fertilizasyon, gebelik oranı ve embriyo kalitesinin ilişkisini araştırmak ayrıca sperm morfolojisi ile erken yarıklanma yüzdesini karşılaştırmaktır. Gereç ve Yöntem: Reprospektif olarak planlanan çalışmaya 60 infertil çift alınmıştır. Azospermik hastalar çalışmaya dahil edilmezken kadın yaşı 40’ın altında tutulmuştur. Sperm morfolojisi hematoksilen yöntemiyle boyanan preperatlar, Kruger morfoloji kriterlerine göre değerlendirilmiştir. Sperm morfolojisi ?%4, normal kabul edilmiştir. Fertilizasyon, 16- 18. saatte 2 pronukleus olarak kabul edilirken, embriyo kalitesi embriyoların 3. günde morfolojik olarak günüyle uyumlu blastomer sayısı, blastomer büyüklüklerinin eşitliği ve fragmentasyon durumuna göre grade 1, 2, 3 olarak klasifiye edilmiştir. Çalışmada, sperm morfolojisi ile fertilizasyon, morfokinezis, embriyo kalitesi, erken yarıklanma ve gebelik oranları değerlendirildi. Bulgular: Normal morfolojili sperm oranı <%4, (n=30; çalışma grubu), ve ?%4, (n=30; kontrol grubu) olmak üzere toplam 60 hastanın verileri istatistiksel değerlendirmeye alındı. Fertilizasyon oranı, normozoospermi grubunda %53, teratozoospermi grubunda %46 bulunmuştur (p>0,01). Bu gruplar embriyo kalitesi olarak karşılaştırıldığında grade 1, 2, 3 embriyo oranlarında, gruplar arasında fark izlenmemiştir (p>0,01). Erken yarıklanma ile sperm morfolojisi arasında anlamlı bir ilişki bulunamamıştır (p>0,01). Gebelik oranları incelendiğinde sonuç, teratozoospermi grubunda %45, normozoospermi grubunda %55’dir (p>0,01). Sonuç: Bu çalışmada, sperm morfolojisi ile fertilizasyon, embriyo kalitesi, erken yarıklanma ve gebelik yüzdeleri arasında anlamlı bir ilişki görülmemiştir.
Aim: To investigate the correlation of embryo quality, fertilization and pregnancy rates with sperm morphology in ICSI (Intracytoplasmic sperm injection) cycles. Also, to compare early cleavage percentage with the sperm morphology. Methods: 60 infertile couples were included in this retrospective study. Azoospermic patients were not included in the study and female age was kept below 40. Sperm preparates were stained with hematoxylin method and morphology was evaluated according to Kruger criteria. Sperm morphology > 4% was accepted as normal. Fertilization was accepted as 2 pronuclei between 16th and 18th hours; embryo quality was classified as grade 1, 2 ,3 depending on the morphology and uniform distribution of blastomeres, and fragmentation on the third day. Fertilization, morphokinesis, embryo quality, early cleavage and pregnancy rates were evaluated according to sperm morphology in the current study. Results: Study group with teratozoospermi (normal morphology <%4, n=30) and control group with normozoospermi (normal morphology ?%4, n=30; kontrol grubu) were evaluated among 60 patients. Fertilization rates were 53% and 40% in the normal and abnormal groups, respectively (p>0,01). Embryo quality were similar among both groups according to grade 1, grade 2 and grade 3 embryos (p>0,01). There was no significant correlation between the early cleavage and sperm morphology (p>0,01). Pregnancy rates were similar in both groups (45% and 55% in study and control groups, respectively (p>0,01). Conclusion: There is no significant relationship between sperm morphology and fertilization, , embryo quality, early cleavage and pregnancy rates in the current study
Aim: To investigate the correlation of embryo quality, fertilization and pregnancy rates with sperm morphology in ICSI (Intracytoplasmic sperm injection) cycles. Also, to compare early cleavage percentage with the sperm morphology. Methods: 60 infertile couples were included in this retrospective study. Azoospermic patients were not included in the study and female age was kept below 40. Sperm preparates were stained with hematoxylin method and morphology was evaluated according to Kruger criteria. Sperm morphology > 4% was accepted as normal. Fertilization was accepted as 2 pronuclei between 16th and 18th hours; embryo quality was classified as grade 1, 2 ,3 depending on the morphology and uniform distribution of blastomeres, and fragmentation on the third day. Fertilization, morphokinesis, embryo quality, early cleavage and pregnancy rates were evaluated according to sperm morphology in the current study. Results: Study group with teratozoospermi (normal morphology <%4, n=30) and control group with normozoospermi (normal morphology ?%4, n=30; kontrol grubu) were evaluated among 60 patients. Fertilization rates were 53% and 40% in the normal and abnormal groups, respectively (p>0,01). Embryo quality were similar among both groups according to grade 1, grade 2 and grade 3 embryos (p>0,01). There was no significant correlation between the early cleavage and sperm morphology (p>0,01). Pregnancy rates were similar in both groups (45% and 55% in study and control groups, respectively (p>0,01). Conclusion: There is no significant relationship between sperm morphology and fertilization, , embryo quality, early cleavage and pregnancy rates in the current study
Açıklama
Anahtar Kelimeler
Sperm morfolojisi, embriyo, erken yarıklanma, Sperm morphology, embryo, early cleavage
Kaynak
Maltepe Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
11
Sayı
1.Şub
Künye
Berberoğlu, Ö., Cıncık, M. ve Selam, B. (2019). Time-lapse (tam zamanli görüntüleme sistemi) ile takip edilen IVF sikluslarinda, teratozoospermi ile normozoospermi vakalarinin embriyo morfokinetiğinin retrospektif karşılaştırılması / Retrospective comparison of embryo morphokinesis between cases of teratozoospermia and normozoospermia in IVF cycles followed by time-lapse imaging system. Maltepe Tıp Dergisi. 11(1), s. 5-9.
