Effect of carnitine on intensive exercise induced decreased glycogen, accumulated lactic acid and morphological changes in skeletal muscle
Küçük Resim Yok
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Türkiye Klinikleri
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Yoğun musküler egzersizde iskelet kasında oluşan değişiklikler ve karnitinin buna etkisini araştırmak amacıyla 80 adet Sprague-Dawley türü yetişkin sıçan kullanıldı. Sıçanlar kontrol, karnitin, egzersiz ve karnitin+egzersiz olmak üzere 4 eşit gruba ayrıldı. Karnitin, 300 mg/kg/gün dozunda olmak üzere 10 gün boyunca, 3 ml serum fizyolojik (SF) içerisinde intraperitoneal yoldan uygulandı. Egzersiz gruplarına enjeksiyonları takiben 30 dk süreyle yüzme egzersizi yaptırıldı. İstatistiksel karşılaştırmada post-ANOVA testi kullanıldı. Deney süreci sonunda iskelet kasından hazırlanan preparatlar morfolojik olarak ışık mikroskobu seviyesinde değerlendirildi. Hematoksilen-Eosin yöntemiyle boyanan kesitlerde egzersiz grubu kas morfolojisinin, karnitin+egzersiz grubuna göre daha fazla bozulduğu gözlendi. Best Carmine ile boyanan preparatlarda ise karnitin+egzersiz grubunun kas glikojen miktarı, egzersiz grubuna göre daha çok olarak izlendi. Öte yandan, sıçanlardan alınan örneklerde, egzersiz grubunda serum serbest karnitin düzeyleri, iskelet kası karnitin düzeyleri ve iskelet kası glikojeni anlamlı olarak azalmış bulunurken (p<0.001), diğer gruplarda normal sınırlar içerisindeydi. Bu bulgular ışığında, karnitinin egzersiz sırasında kullanılan bir madde olduğunu ve yoğun musküler egzersiz öncesinde verildiği takdirde, kas karnitini ve glikojen depolarını koruyabileceğini söylemek mümkündür.
80 adult male Sprague-Dawley rats were used to investigate the changes in skeletal muscle induced by intensive muscular exercise and the effects of carnitine. The rats were divided into 4 equal groups as control, carnitine, exercise and carnitine+exercise. Carnitine was administered intraperitoneally, at a dose of 300 mg/kg/day dissolved in 3 cc saline, for 10 days. The same amount of saline was injected to the exercise and control groups. Swimming exercise was performed for 30 minutes after injections. Statistical comparisons were made by using post-ANOVA test. Serum free carnitine, muscle carnitine and glycogen levels decreased significantly in exercise group. In the other groups these parameters found to remain in physiological levels. Skeletal muscle preparations were examined morphologically by light microscope. Samples dyed by Haematoxylen-Eosin showed that muscle morphology in exercise group altered more than carnitine+exercise group. Also, muscle glycogen content was seen more in exercise group than carnitine+ exercise group, by Best Carmine dye. In conclusion, it is possible to say that carnitine is a substance used during exercise and carnitine administration before intensive muscular exercise may prevent the decrease of muscle carnitine and glycogen contents.
80 adult male Sprague-Dawley rats were used to investigate the changes in skeletal muscle induced by intensive muscular exercise and the effects of carnitine. The rats were divided into 4 equal groups as control, carnitine, exercise and carnitine+exercise. Carnitine was administered intraperitoneally, at a dose of 300 mg/kg/day dissolved in 3 cc saline, for 10 days. The same amount of saline was injected to the exercise and control groups. Swimming exercise was performed for 30 minutes after injections. Statistical comparisons were made by using post-ANOVA test. Serum free carnitine, muscle carnitine and glycogen levels decreased significantly in exercise group. In the other groups these parameters found to remain in physiological levels. Skeletal muscle preparations were examined morphologically by light microscope. Samples dyed by Haematoxylen-Eosin showed that muscle morphology in exercise group altered more than carnitine+exercise group. Also, muscle glycogen content was seen more in exercise group than carnitine+ exercise group, by Best Carmine dye. In conclusion, it is possible to say that carnitine is a substance used during exercise and carnitine administration before intensive muscular exercise may prevent the decrease of muscle carnitine and glycogen contents.
Açıklama
Anahtar Kelimeler
Karnitin, Kas, İskelet, Varyans analizi, Glikojen, Sıçan, Sprague-Dawley, Egzersiz, Muscle, Skeletal, Carnitine, Analysis of Variance, Exercise, Rats, Sprague-Dawley, Glycogen
Kaynak
Türkiye Klinikleri Tıp Bilimleri Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
20
Sayı
6
Künye
Coşkun, Ö., Öter, Ş., Korkmaz, A., Sezen, Ş., Öztaş, E. ve Cıncık, M. (2000). Effect of carnitine on intensive exercise induced decreased glycogen, accumulated lactic acid and morphological changes in skeletal muscle / Yoğun egzersiz ile iskelet kasında oluşan glikojen azalması, laktik asit birikmesi ve morfolojik değişikliklere, karnitinin etkisi. Türkiye Klinikleri Tıp Bilimleri Dergisi, Türkiye Klinikleri. 20(6), s. 325-333.
