Different approaches for breast cancer: voltage gated potassium channels and micrornas
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Ankara University Faculty of Sciences
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Micro RNAs and voltage-gated potassium channels (VGPCs) both play critical roles in the development of cancer. We aimed to reveal the diversity of miR-126/126*, which effects angiogenesis and vascular development through the inhibition of VGPCs. In this study, potassium channel inhibitors, including tetraethylammonium (5mM), 4-aminopyridine (5mM), margatoxin (1µM) and astemizole (2µM), were applied to MCF-7 and MDA-MB-231 breast cancer cell lines. After totally isolating RNA from the cells, Real-Time Polymerase Chain Reaction was used in order to identify gene expressions. OneWay ANOVA was used for variation analyses, while Tukey HSD and Tamhane were used to assess whether multiple comparisons were statistically significant (P < 0.001). Our results showed an increase in miR-126/126* expressions after the channel inhibition of MCF-7 and MDA-MB-231 cell lines (P < 0.001). miR-126/126* expressions were increased using TEA, 4-AP and astemizole in both cell lines. miR-126/126* expressions were only increased through the use of margatoxin in MCF-7. miR-126/126* may interact with voltage-gated potassium channels. In our study, the inhibition of K channels using K channel blockers resulted in an increase of miR-126/126* expression. Therefore, our data suggested that there could be another perspective between K channels and non-coding RNAs in the development of breast cancer.
Description
Keywords
Breast Cancer, miR-126/126*, Voltage-Gated Potassium Channels
Journal or Series
Communications Faculty of Sciences University of Ankara Series C-Biology
WoS Q Value
Scopus Q Value
Volume
24
Issue
1.Şub
Citation
Öner, Ç., Turgut Coşan, D. ve Çolak, E. (2015). Different approaches for breast cancer: voltage gated potassium channels and micrornas. Communications Faculty of Sciences University of Ankara Series C-Biology, Ankara University Faculty of Sciences. 24(1-2), s. 1-17.
