Rescue IVM of denuded GV- and mi-stage oocytes of premenopausal rats with oncostatin M, insulin-like growth factor ı, and growth hormone

Küçük Resim Yok

Tarih

2022

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Life

Erişim Hakkı

info:eu-repo/semantics/openAccess

Araştırma projeleri

Organizasyon Birimleri

Dergi sayısı

Özet

Immature oocytes are retrieved and matured through in vitro maturation (IVM). Maturation, fertilization rates, and embryo development via IVM are all lower than those found in vitro fertilization (IVF) cycles. We investigated the effects of oncostatin M (OSM), insulin-like growth factor-1 (IGF-I), and growth hormone (GH) in rescue IVM. A total of 111 germinal vesicle (GV) and 17 metaphase I (MI) oocytes were obtained after conventional IVF from 28 female Wistar albino rats. Denuded immature oocytes were cultured in maturation medium supplemented with OSM, IGF-1, or GH. The quantities of metaphase II (MII) oocytes matured from the GV stage were 17 of 30 (56.6%), 15 of 28 (53.5%), 10 of 30 (33.3%), and 7 of 23 (30.3%), in control, OSM, IGF-I, and GH groups, respectively. Maturation rates in control and OSM groups were higher than those in IGF-I and GH groups (p = 0.001). The quantities of MII oocytes matured from MI stage were 7 of 7 (100%), 4 of 4 (100%), 1 of 1 (100%), and 1 of 5 (20%) in control, OSM, IGF-I, and GH groups, respectively. Maturation rates from MI to MII stages in control, OSM, and IGF-I groups were higher than those in the GH group (p = 0.004). Acceptable maturation rates are observed with OSM in rat oocytes in rescue IVM.

Açıklama

Anahtar Kelimeler

In-vitro maturation, Germinal vesicle, Oncostatin M, Insulin-like growth factor-1, Growth hormone, MII oocyte, Rescue in vitro maturation

Kaynak

Life

WoS Q Değeri

Scopus Q Değeri

Cilt

12

Sayı

Künye

Akdemir, Y., Cakil, Y.D., Selam, B., Sitar, M.E., Cincik, M. (2022). Rescue IVM of denuded GV- and mi-stage oocytes of premenopausal rats with oncostatin M, insulin-like growth factor ı, and growth hormone. Life, 12, p.1247.