Effects of leptin on the viability of MCF-7 and T47D cells at different glucose concentrations

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dc.contributor.authorDemirel, P.B.
dc.contributor.authorDogan, S.
dc.contributor.authorOzorhan, U.
dc.contributor.authorTuna, B.G.
dc.contributor.authorSchuster, T.F.
dc.contributor.authorCleary, M.P.
dc.date.accessioned2024-07-12T21:40:03Z
dc.date.available2024-07-12T21:40:03Z
dc.date.issued2020en_US
dc.department[Belirlenecek]en_US
dc.description.abstractObesity is associated with increased risk of breast cancer. Leptin is a well-known factor involved in obesity and its serum levels are increased in breast cancer. Hyperglycemia is another significant risk factor for breast cancer. Consistently, high glucose induces proliferation and invasion of breast cancer cells and in-vivo calorie restriction reduce tumorigenesis in rodent models. The aim of this study was to investigate the effect of leptin on the viability and mode of cell death in breast cancer cells incubated in different glucose concentrations to represent caloric restriction. For this purpose, MCF-7 and T47D breast cancer cells incubated in different glucose concentrations for a total of 72 hours were treated with or without leptin either for one hour or 24 hours and the ratio of apoptotic, necrotic and alive cells were analyzed by flow cytometry. Our data revealed that glucose incubation significantly decreased apoptosis and necrosis, while increasing viability in both cell lines in a dose dependent manner. One-hour leptin treatment significantly decreased viability, and increased apoptosis but did not significantly affect necrosis in T47D cells incubated in 2.5 mM glucose. In MCF-7 cells, one-hour leptin incubation significantly increased necrosis but its effects on apoptosis and viability were not significant. In conclusion, although glucose induces cell death by apoptosis and necrosis in T47D and MCF-7 cells respectively in a dose dependent manner, the overallviability is still increased in both cell lines. One-hour leptin treatment reverses the effect of low glucose incubation on apoptosis of T47D and necrosis of MCF-7 cells. Moreover, the effect of one-hour leptin treatment on apoptosis or necrosis is significantly higher than that of 24-hour leptin treatment. © 2020 Ondokuz Mayis Universitesi. All rights reserved.en_US
dc.description.sponsorshipBreast Cancer Research Foundation, BCRF; Hormel Foundationen_US
dc.description.sponsorshipThis work was supported by grants NIH-CA101858, CA157012 (MPC) and The Breast Cancer Research Foundation and The Hormel Foundation, Minnesota. The authors thank summer intern undergraduate student, Swetha S. Ramanan, for helping us maintaining the cell culture.en_US
dc.identifier.doi10.5835/jecm.omu.37.04.003
dc.identifier.endpage125en_US
dc.identifier.issn1309-4483
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85105507549en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage119en_US
dc.identifier.urihttps://doi.org/10.5835/jecm.omu.37.04.003
dc.identifier.urihttps://hdl.handle.net/20.500.12415/7115
dc.identifier.volume37en_US
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherOndokuz Mayis Universitesien_US
dc.relation.ispartofJournal of Experimental and Clinical Medicine (Turkey)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY04839
dc.subjectCalorie Restrictionen_US
dc.subjectCancer Cell Viabilityen_US
dc.subjectGlucoseen_US
dc.subjectLeptinen_US
dc.subjectMcf-7 T47den_US
dc.titleEffects of leptin on the viability of MCF-7 and T47D cells at different glucose concentrationsen_US
dc.typeArticle
dspace.entity.typePublication

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