The effect of the iNOS inhibitor S-methylisothiourea and hyperbaric oxygen treatment on radiation colitis in rats

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dc.contributor.authorDemirci H.
dc.contributor.authorPolat Z.
dc.contributor.authorUygun A.
dc.contributor.authorKadayifci A.
dc.contributor.authorSager O.
dc.contributor.authorOzturk K.
dc.contributor.authorKantarcioglu M.
dc.contributor.authorSahiner F.
dc.contributor.authorCaliskan B.
dc.contributor.authorKarslioglu Y.
dc.contributor.authorOzler M.
dc.contributor.authorOzel M.
dc.contributor.authorErgun H.
dc.contributor.authorOzturk O.
dc.contributor.authorBeyzadeoglu M.
dc.contributor.authorBagci S.
dc.date.accessioned2024-07-12T21:44:17Z
dc.date.available2024-07-12T21:44:17Z
dc.date.issued2016en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractIntroduction: External radiotherapy is one of the main treatment modalities for a variety of malignancies. However, the lower gastrointestinal tract is sensitive to the ionizing radiation. Hyperbaric oxygen treatment (HOT) has been suggested as a viable treatment for refractory radiation colitis, but the effect of S-Methylisothiourea (SMT) in the radiation colitis have not reported. Aim: To investigate the effect of SMT, HOT and the combination of both in an acute radiation-induced enterocolitis model. Methods: Sixty Sprague-Dawley rats were divided randomly into five equal groups. A single dose of gamma irradiation (25 Gy) was administered through the colorectal region to anesthetized rats. In the control group, we applied 2 ml of saline solution intraperitoneally for five days. In the HOT group, 100-per-cent oxygen at 2.5 atm pressure was applied for five days. In the SMT group, 10 mg/kg/day of SMT was applied intraperitoneally for five days. In the HOT+SMT group, HOT and SMT were both applied in the same dosages as in the preceding two groups. At the end of five days, the rats were sacrificed and colon samples were collected for histological grading. Blood samples were collected to test for: tumor necrosis factor-? (TNF-?), interleukin-10 (IL-10), IL-1ß, transforming growth factor-ß (TGF-ß) and intercellular adhesion molecule-1 (ICAM-1) mRNA. Results: The TNF-?, IL-1ß, IL-10 and TGF-ß levels were reduced by SMT, HOT and HOT+SMT applications (p < 0.05). However ICAM-1 mRNA levels were not significantly lower (p:0.19). The microscopic scores differed significantly between the SMT, HOT and HOT+SMT groups and the control group. There was significant improvement histologically, especially in the HOT+SMT group. When we compared the weight of the rats before and after the study, weight loss was significantly lower in the SMT, HOT and HOT+SMT groups compared with the control group (p < 0.05). Conclusions: HOT and SMT together were significantly more effective in preventing weight loss and in reducing inflammation and the severity of colitis histology when compared with HOT and SMT separately. (Acta gastro enterol. belg., 2015, 78, 8-13). © 2016, Universa Press. All rights reserved.en_US
dc.identifier.endpage13en_US
dc.identifier.issn1784-3227
dc.identifier.issue1en_US
dc.identifier.pmid26852757en_US
dc.identifier.scopus2-s2.0-84957968151en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage8en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/7707
dc.identifier.volume79en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherUniversa Pressen_US
dc.relation.ispartofActa Gastro-Enterologica Belgicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY00043
dc.subjectHyperbaric oxygen treatmenten_US
dc.subjectInducible nitric oxide synthase (iNOS) inhibitoren_US
dc.subjectRadiation colitisen_US
dc.subjectS-Methylisothioureaen_US
dc.titleThe effect of the iNOS inhibitor S-methylisothiourea and hyperbaric oxygen treatment on radiation colitis in ratsen_US
dc.typeArticle
dspace.entity.typePublication

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