Detection of hepatitis C virus RNA in paraffin-embedded tissues from patients with non-Hodgkin's lymphoma

Basit Öğe Kaydı
dc.authorid0000-0002-8335-1927en_US
dc.contributor.authorPaydaş, Semra
dc.contributor.authorErgin, Melek
dc.contributor.authorTanrıverdi, Kahraman
dc.contributor.authorYavuz, Sinan
dc.contributor.authorDisel, Umut
dc.contributor.authorKılıç, Nil Banu
dc.contributor.authorErdoğan, Şeyda
dc.contributor.authorŞahin, Berksoy
dc.contributor.authorTunçer, İlhan
dc.contributor.authorBurgut, Hüseyin Refik
dc.date.accessioned2024-07-12T21:01:27Z
dc.date.available2024-07-12T21:01:27Z
dc.date.issued2004en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractThe aim of this study is to detect the possible role of hepatitis C Virus (HCV) in lymphomagenesis. HCV-RNA and anti-HCV antibodies were studied in tissue and serum samples taken from patients with non-Hodgkin's Lymphoma (NHL). The prevalence of HCV, the clinical presentation of these cases, and association with histologic subtypes were determined. RT-PCR was used to detect the HCV-RNA in serum and tissue samples. The anti-HCV antibodies were tested with microparticle enzyme immunoassay. Immunohistochemistry with the ABC method was used to detect the HCV core protein in HCV-RNA(+) cases. RNA could be detected in 30 of 35 cases, and other tests were performed in these 30 samples. HCV-RNA was detected in 11 tissue samples (11/30, 37%). HCV core protein was studied in 10 of 11 HCV-RNA(+) cases, and 1-3% nuclear staining was found in only 2 samples. Serologically, HCV-RNA was detected in 7 of 30 samples (23.3%) and anti-HCV antibody was detected in 3 of 30 samples (10%). Detection of HCV-RNA in 37% of the lymphoma tissue samples suggests that HCV may have a role or is a contributing factor in the pathogenesis of lymphoma. The very low HCV core protein in lymphoma tissues may be due to the low viral load in lymphoid tissues and/or higher sensitivity of the PCR method. Detection of anti-HCV antibody in only three cases may be associated with undetectable levels of antibodies due to the immune deficiency in cases with NHL.en_US
dc.identifier.citationPaydaş, S., Ergin, M., Tanrıverdi, K., Yavuz, S., Disel, U., Kılıç, N. B., Erdoğan, Ş., Şahin, B., Tunçer, İ. ve Burgut, H. R. (2004). Detection of hepatitis C virus RNA in paraffin-embedded tissues from patients with non-Hodgkin's lymphoma. American Journal of Hematology Journal. 76(3), s. 252-257.en_US
dc.identifier.endpage257en_US
dc.identifier.issue3en_US
dc.identifier.startpage252en_US
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/15224361/
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3504
dc.identifier.volume76en_US
dc.institutionauthorBurgut, Hüseyin Refik
dc.language.isoenen_US
dc.publisherNational Center for Biotechnology Informationen_US
dc.relation.ispartofAmerican Journal of Hematology Journalen_US
dc.relation.isversionof10.1002/ajh.20092en_US
dc.relation.publicationcategoryUlusal Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY00169
dc.titleDetection of hepatitis C virus RNA in paraffin-embedded tissues from patients with non-Hodgkin's lymphomaen_US
dc.typeArticle
dspace.entity.typePublication

Dosyalar

Koleksiyon

Tıp Fakültesi Koleksiyonu