Monitoring T-Cell Kinetics in the Early Recovery Period of Lung Transplantation Cases by Copy Number Levels of T-Cell Receptor Excision Circle

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dc.contributor.authorFatma, T.A.
dc.contributor.authorAkbulut, Z.
dc.contributor.authorVayvada, M.
dc.contributor.authorKalamanoğlu, Balci M.
dc.contributor.authorYegi?Nsu, A.
dc.contributor.authorYanikkaya, Demi?rel G.
dc.contributor.authorAsim, Kutlu C.
dc.date.accessioned2024-07-12T21:40:17Z
dc.date.available2024-07-12T21:40:17Z
dc.date.issued2023en_US
dc.department[Belirlenecek]en_US
dc.description.abstractBackground/Aim: Lung transplantation is a lifesaving procedure for patients with end-stage lung diseases. T-Cell receptor excision circle (TREC) is circular DNA produced during T-cell receptor gene rearrangement in the thymus and indicates naive T-cell migration from the thymus. Therefore, its levels represent thymic T-cell output. Post-transplant lymphocyte kinetics correlate with graft tolerance. The aim of this study was to investigate T-lymphocyte kinetics in the early recovery period after lung transplantation. For this purpose, copy numbers of TREC were determined in patients with a lung transplant. In addition, TREC copy numbers were evaluated according to age, diagnosis and the forced expiratory volume in 1 second (FEV1) of lung transplant patients. Materials and Methods: Peripheral blood samples were taken from patients aged 23 to 59 years who underwent lung transplantation at the Thoracic Surgery Clinic, Kartal-Koşuyolu High Specialization Educational and Research Hospital. This study included peripheral blood samples from 11 lung transplant patients (comprising four with chronic obstructive pulmonary disease, three with idiopathic pulmonary fibrosis, one with cystic fibrosis, one with silicosis and two with bronchiectasis; three females in total). Samples were taken at three different timepoints: Before transplant, and 24 hours and 7 days post transplant. TREC copy numbers were analyzed with real time reverse transcriptase–polymerase chain reaction. Results: Post-transplant TREC numbers and density values were higher compared to pre-transplant values, although these differences were statistically insignificant. TREC copy numbers were found to be significantly higher in patients younger than 45 years compared to patients older than 45 years. At 24 hours after the transplant, the average TREC copy number/peripheral blood mononuclear cells of the cases with an FEV1 value of or below 50% was found to be statistically significantly higher than that of cases with an FEV1 value above 50% (p=0.046). There was no statistically significant difference in TREC copy numbers between male and female patients or by diagnostic group. Conclusion: TREC copy numbers can be evaluated as a prognostic marker for lung transplantation. There is a need for multicenter studies with more patients. © 2023 International Institute of Anticancer Research. All rights reserved.en_US
dc.identifier.doi10.21873/invivo.13081
dc.identifier.endpage319en_US
dc.identifier.issn0258-851X
dc.identifier.issue1en_US
dc.identifier.pmid36593057en_US
dc.identifier.scopus2-s2.0-85145492208en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage310en_US
dc.identifier.urihttps://doi.org/10.21873/invivo.13081
dc.identifier.urihttps://hdl.handle.net/20.500.12415/7219
dc.identifier.volume37en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherInternational Institute of Anticancer Researchen_US
dc.relation.ispartofIn Vivoen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY05129
dc.subjectImmunologyen_US
dc.subjectLung Transplantationen_US
dc.subjectT Cell Kineticsen_US
dc.subjectTrecen_US
dc.titleMonitoring T-Cell Kinetics in the Early Recovery Period of Lung Transplantation Cases by Copy Number Levels of T-Cell Receptor Excision Circleen_US
dc.typeArticle
dspace.entity.typePublication

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