LEPTIN RECEPTORS EXPRESSION IN MAMMARY TUMORS AND MAMMARY FAT PAD OF TRANSGENIC MAMMARY CANCER MOUSE MODEL

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dc.contributor.authorYılmaz, E.
dc.contributor.authorThomas, P.B.
dc.contributor.authorTuna, B.G.
dc.contributor.authorCleary, M.P.
dc.contributor.authorDogan, S.
dc.date.accessioned2024-07-12T21:40:14Z
dc.date.available2024-07-12T21:40:14Z
dc.date.issued2022en_US
dc.department[Belirlenecek]en_US
dc.description.abstractBackground: Leptin is an adipokine encoded by the Ob (obese) gene and predominantly produced by adipocytes. The roles of both leptin and leptin receptor (ObR) in numerous pathophysiological conditions including mammary tumor (MT) development have been reported. Aim: To examine protein expression levels of leptin and its receptors (ObR) including the long form, ObRb, in MT tissue and mammary fat pad of a transgenic mammary cancer mouse model. Further, we investigated whether the effects of leptin on MT development are systemic or local. Materials and Methods: MMTV-TGF-? transgenic female mice were fed ad libitum from week 10 up to week 74. Protein expression levels of leptin, ObR, and ObRb were measured in the mammary tissue samples of 74-week old MMTV-TGF-? mice with and without MT (MT-positive/MT-negative) by Western blot analysis. Serum leptin levels were measured by using the mouse adipokine LINCOplex kit 96-well plate assay. Results: Protein expression levels of ObRb were significantly lower in MT as compared to control tissue of mammary gland. In addition, protein expression levels of leptin were significantly higher in the MT tissue of MT-positive mice compared to control tissue of MT-negative mice. However, ObR protein expression levels in tissues of mice with and without MT were similar. Serum leptin levels at different ages were not significantly different between the two groups. Conclusion: Leptin and ObRb in the mammary tissue may play a critical role in the mammary cancer development, while contribution of short ObR isoform may be less important. Copyright © Experimental Oncology, 2022.en_US
dc.description.sponsorshipNational Institutes of Health, NIH: CA101858; Breast Cancer Research Foundation, BCRF; Hormel Foundationen_US
dc.description.sponsorshipThis work was supported by NIH grant (CA101858) (MPC), and by The Breast Cancer Research Foundation and The Hormel Foundation. The authors thank Joseph P. Grande for pathological analyses. The authors also thank undergrad student Rebecca N. LeVan for her technical assistance and Amy Snider, Laura Hamersma, Michelle Jacobson, Miranda Goff and Lynn Leraaen for animal care. Finally, the authors are grateful to Dr. Olga Rogozina, Melissa J. L. Bonorden and Nancy K. Mizuno for maintaining the breeding colony and genotyping of the mice.en_US
dc.identifier.doi10.32471/exp-oncology.2312-8852.vol-44-no-4.18941
dc.identifier.endpage280en_US
dc.identifier.issn1812-9269
dc.identifier.issue4en_US
dc.identifier.pmid36811542en_US
dc.identifier.scopus2-s2.0-85148551189en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage272en_US
dc.identifier.urihttps://doi.org/10.32471/exp-oncology.2312-8852.vol-44-no-4.18941
dc.identifier.urihttps://hdl.handle.net/20.500.12415/7179
dc.identifier.volume44en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherMorion LLCen_US
dc.relation.ispartofExperimental Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY05047
dc.subjectLeptinen_US
dc.subjectLeptin Receptoren_US
dc.subjectMammary Fat Paden_US
dc.subjectMammary Tumoren_US
dc.subjectMmtv-Tgf-? Miceen_US
dc.titleLEPTIN RECEPTORS EXPRESSION IN MAMMARY TUMORS AND MAMMARY FAT PAD OF TRANSGENIC MAMMARY CANCER MOUSE MODELen_US
dc.typeArticle
dspace.entity.typePublication

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