Low-dose cisplatin exposure and snail, vimentin, e-cadherin expression in hepg2 cell line

Basit Öğe Kaydı
dc.authorid0000-0002-4605-1167en_US
dc.authorid0000-0001-9801-1506en_US
dc.authorid0000-0002-4474-7371en_US
dc.authorid0000-0002-3060-1507en_US
dc.date.accessioned2024-07-12T21:04:14Z
dc.date.available2024-07-12T21:04:14Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractCisplatin, the first platinum compound approved for cancer treatment, is widely used in the treatment of various cancers including hepatocellular carcinoma (HCC). HCC incidence rates rise globally. Epithelial mesenchymal transition (EMT) is implicated in cancer invasion and metastasis, which are associated with increased mortality. Cisplatin dose might influence cancer invasion and metastatic behavior of the cells. The aim of the study was to investigate the effect of low-dose cisplatin treatment on EMT- related changes in HepG2 cells. Following treatment with 4 µM cisplatin, HepG2 cells were evaluated morphologically. Gene expression of E-cadherin, Vimentin, Snail1 was assessed by quantitative PCR. Immunofluorescence analyses of NA-K ATPase were performed. Although the low-dose cisplatin treated cells exhibited a more stretched morphology, no statistical difference was detected in gene expression of E-cadherin, Vimentin, Snail1 and immunofluorescence of NA-K ATPase. Findings on low-dose cisplatin effects in HepG2 might contribute to the knowledge of antineoplastic inefficacy by further understanding the molecular mechanisms of drug action.en_US
dc.identifier.citationDönmez Çakıl, Y., Akbulut, Z., Aktaş, R.G. ve Özünal, Z.G. (2022). Low-dose cisplatin exposure and snail, vimentin, e-cadherin expression in hepg2 cell line / Exposición a dosis bajas de cisplatino y expresión de snail, vimentina, e-cadherina en línea celular hepg2. International Journal of Medical and Surgical Sciences, Universidad Autonoma de Chile. 9(2), s. 1-9.en_US
dc.identifier.endpage9en_US
dc.identifier.issn0719-532X
dc.identifier.issue2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://revistas.uautonoma.cl/index.php/ijmss/article/view/1864
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3768
dc.identifier.volume9en_US
dc.institutionauthorDönmez Çakıl, Yaprak
dc.institutionauthorAkbulut, Zeynep
dc.institutionauthorAktaş, Ranan Gülhan
dc.institutionauthorÖzünal, Zeynep Güneş
dc.language.isoenen_US
dc.publisherUniversidad Autonoma de Chileen_US
dc.relation.ispartofInternational Journal of Medical and Surgical Sciencesen_US
dc.relation.isversionof10.32457/ijmss.v9i2.1864en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY01662
dc.subjectCisplatinen_US
dc.subjectSNAILen_US
dc.subjectVimentinen_US
dc.subjectE-cadherinen_US
dc.subjectEpithelial mesenchymal transitionen_US
dc.titleLow-dose cisplatin exposure and snail, vimentin, e-cadherin expression in hepg2 cell lineen_US
dc.title.alternativeExposición a dosis bajas de cisplatino y expresión de snail, vimentina, e-cadherina en línea celular hepg2en_US
dc.typeArticle
dspace.entity.typePublication

Dosyalar

Koleksiyon

Tıp Fakültesi Koleksiyonu