A Frequently Overlooked Bacteria in Clinical Microbiology Laboratories: Arcanobacterium haemolyticum

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dc.contributor.authorBalikci, Ahmet
dc.contributor.authorTopkaya, Aynur E.
dc.contributor.authorBelas, Zeliha
dc.date.accessioned2024-07-12T21:52:10Z
dc.date.available2024-07-12T21:52:10Z
dc.date.issued2011en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractArcanobacterium haemolyticum, previously known as Corynebacterium haemolyticum, is a facultative anaerobic, gram-positive bacillus with negative catalase and positive CAMP inhibition test results. It may be the causative agent of about 0.5-3% of acute bacterial pharyngitis especially in children and young adults. Since growth of A.haemolyticum is usually inhibited by flora members and since it slowly develops hemolysis in sheep blood agar and its colony morphology resembles beta-hemolytic streptococci, it is frequently overlooked in the evaluation of throat cultures. The aims of this study were to investigate the isolation frequency of A.haemolyticum from the throat cultures of pediatric patients by using both sheep and human blood agar media, and to evaluate the performances of those media for the identification of A.haemolyticum. A total of 355 patients (median age: 7 years) who were admitted to pediatric outpatient clinics with the symptoms of tonsillopharyngitis between March-July 2010 period, were included in the study. Swab samples obtained from tonsils and posterior oropharynx were inoculated into a divided plate which contained 5% sheep blood agar in one half and 5% human blood agar in the other half. After incubation in 5% CO(2) at 37 degrees C, the beta-hemolytic colonies with a microscopic morphology of gram-positive bacilli were further evaluated on 24, 48 and 72(th) hours. Identification of A.haemolyticum was based on negative catalase test, positive reverse CAMP test and biochemical characteristics obtained by API-Coryne (bioMerieux, France) identification system. In our study, beta-hemolytic colonies were detected in the throat cultures of 56 (16%) patients, of which 14% (49/355) were identified as beta-hemolytic streptococci (46 group A, 2 group G, 1 group C), and 2% (7/355) were identified as A.haemolyticum. All of the A.haemolyticum isolates were characterized by the production of beta-hemolysis in human blood agar at 24 hours, while the beta-hemolysis generation time in sheep blood agar was 48 hours for four isolates and 72 hours for three isolates. A.haemolyticum was identified in 2% of children with tonsillopharyngitis during the five months study period in spring/summer. All of the strains were isolated at human blood agar in 24 hours. Thus, in order to isolate A.haemolyticum in routine throat cultures, sheep blood agar plates together with human blood agar plates should be used in clinical microbiology laboratories.en_US
dc.identifier.endpage540en_US
dc.identifier.issn0374-9096
dc.identifier.issue3en_US
dc.identifier.pmid21935787en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage535en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8352
dc.identifier.volume45en_US
dc.identifier.wosWOS:000294879100017en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isotren_US
dc.publisherANKARA MICROBIOLOGY SOCen_US
dc.relation.ispartofMIKROBIYOLOJI BULTENIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY02927
dc.subjectArcanobacterium haemolyticumen_US
dc.subjectpharyngitisen_US
dc.subjectbeta-hemolysisen_US
dc.subjecthuman blood agaren_US
dc.subjectlaboratory diagnosisen_US
dc.titleA Frequently Overlooked Bacteria in Clinical Microbiology Laboratories: Arcanobacterium haemolyticumen_US
dc.typeArticle
dspace.entity.typePublication

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