Development of a hepatitis B virus carrier transgenic mice model

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dc.authorid0000-0001-5291-8620en_US
dc.contributor.authorCaner, Muege
dc.contributor.authorArat, Sezen
dc.contributor.authorBircan, Rifat
dc.date.accessioned2024-07-12T21:52:08Z
dc.date.available2024-07-12T21:52:08Z
dc.date.issued2008en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractThe studies for the development of transgenic mice models which provide important profits for the studies concerning immunopathogenesis of hepatitis B virus (HBV) infections are in progress since 20 years. For this purpose different lineages bearing whole HBV genome or selected viral genes have been developed and their usage in clarifying the HBV replication and pathogenesis mechanisms have been emphasized. The aim of this study was to develop and breed a HBV carrier mice model. In the study the full HBV genome has been transferred to mouse embryos by microinjection procedure. Following transgenic manipulation, the HBV carriers among the daughter mice have been detected by molecular methods in which HBV-DNA replication and expression have been shown. The manipulations for transgene transfers have been performed in TUBITAK Marmara Research Center Transgene Laboratory, Gebze, istanbul. The HBV-DNA carrier mice have been demonstrated by polymerase chain reaction (PCR) using the DNA samples obtained from tail tissues and also by dot-blot hybridization of the mice sera. Integrated HBV-DNA has been detected by applying in-situ hybridization to the liver tissue sections. HBV-DNA expression has been shown by reverse transcriptase PCR method with total RNA molecules that have been isolated from the liver tissues of the HBV-DNA carrier mice. HBsAg has been detected in the liver by immunohistochemical method, and HBsAg and HBeAg have additionally been demonstrated by ELISA. HBV genome, expression of the genome and the expression products have been determined in approximately 10% of the mice of which HBV-DNA have been transferred. By inbreeding heterozygote carrier mice, homozygote HBV transgenic mice line have been obtained. These HBV transgenic mice are the first lineages developed in our country. It is hopefully thought that this HBV carrier transgenic mouse model may contribute to the studies on the pathogenesis of HBV infections which are important health problems in the world as well as in Turkey.en_US
dc.identifier.endpage81en_US
dc.identifier.issn0374-9096
dc.identifier.issue1en_US
dc.identifier.pmid18444564en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage71en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8350
dc.identifier.volume42en_US
dc.identifier.wosWOS:000253792400008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isotren_US
dc.publisherANKARA MICROBIOLOGY SOCen_US
dc.relation.ispartofMIKROBIYOLOJI BULTENIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY02925
dc.subjectHBV infectionen_US
dc.subjectHBV carrying transgenic miceen_US
dc.titleDevelopment of a hepatitis B virus carrier transgenic mice modelen_US
dc.typeArticle
dspace.entity.typePublication

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