The effect of androgens on proinflammatory cytokine secretion from human ocular surface epithelial cells

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dc.authorid0000-0003-3771-3277en_US
dc.contributor.authorMarangoz, Deniz
dc.contributor.authorÖner, Çağrı
dc.contributor.authorSchicht, Martin
dc.contributor.authorTurgut Coşan, Didem
dc.contributor.authorPaulsen, Friedrich
dc.contributor.authorYıldız, Erdost
dc.contributor.authorZibandeh, Noushin
dc.contributor.authorŞahin, Afsun
dc.date.accessioned2024-07-12T21:10:29Z
dc.date.available2024-07-12T21:10:29Z
dc.date.issued2021en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractPurpose: The purpose of this study is to explore the effects of dihydrotestosterone (DHT) on lipopolysaccharide (LPS)-induced proinflammatory cytokine release in human ocular surface epithelial cells exposed to LPS and LPS-binding protein (LBP). Methods: Immortalized human corneal, conjunctival, and meibomian gland epithelial cells were cultured in keratinocyte-free medium. After confluency, they were exposed to a stratification medium Dulbecco's modified Eagle medium (DMEM)/F12 in the presence of fetal bovine serum and were exposed to vehicle, LPS + LBP, or DHT. Culture media were processed for multiplex-bead analysis of specific proinflammatory cytokines including interferon (IFN)-?, tumor necrosis factor (TNF)-?, interleukin (IL)-2, IL-4, IL-8, IL-6, IL-10, IL-1?, vascular endothelial growth factor (VEGF)-A. Cytokine concentrations were compared by analysis of variance with Tukey post hoc testing. p < 0.05 was considered statistically significant. Results: The results are LPS + LBP-induced the secretion of IFN-?, IL-6, IL-10, IL-1?, VEGF-A cytokines in corneal epithelial cells; TNF-?, IL-2, IL-8, IL-6, IL-1?, VEGF-A cytokines in conjunctival epithelial cells; and IL-8, IL-6, IL-1?, VEGF-A cytokines in meibomian gland epithelial cells. When these LPS + LBP-stimulated cells were exposed to DHT for 2 days, it was found that DHT suppressed the secretion of IL-6, IL-10, IL-1?, VEGF-A cytokines in corneal epithelial cells; TNF-?, IL-6, IL-1?, VEGF-A cytokines in conjunctival epithelial cells; and IL-6, IL-1?, VEGF-A cytokines in meibomian gland epithelial cells. Conclusion: LPS + LBP is shown to induce the secretion of certain proinflammatory cytokines from ocular surface and adnexal epithelial cells. DHT showed anti-inflammatory activity by suppressing some of those cytokines in these cell lines.en_US
dc.identifier.citationMarnagoz, D., Öner, Ç., Schicht, M. vd. (2021). The effect of androgens on proinflammatory cytokine secretion from human ocular surface epithelial cells. Ocular Immunology and Inflammation, Taylor & Francis Online. 29(3), s. 546-554.en_US
dc.identifier.endpage554en_US
dc.identifier.issn1744-5078
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage546en_US
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/09273948.2019.1686155
dc.identifier.urihttps://hdl.handle.net/20.500.12415/4256
dc.identifier.volume29en_US
dc.institutionauthorÖner, Çağrı
dc.language.isoenen_US
dc.publisherTaylor & Francis Onlineen_US
dc.relation.ispartofOcular Immunology and Inflammationen_US
dc.relation.isversionof10.1080/09273948.2019.1686155en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY03154
dc.subjectDihydrotestosteroneen_US
dc.subjectocular surface epitheliumen_US
dc.subjectproinflammatory cytokineen_US
dc.subjectlipopolysaccharideen_US
dc.subjectcell cultureen_US
dc.titleThe effect of androgens on proinflammatory cytokine secretion from human ocular surface epithelial cellsen_US
dc.typeArticle
dspace.entity.typePublication

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