Examination of the effect of ovarian radiation injury induced by hysterosalpingography on ovarian proliferating cell nuclear antigen and the radioprotective effect of amifostine: an experimental study

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dc.authorid0000-0002-0208-8929en_US
dc.authorid0000-0003-2635-7158en_US
dc.contributor.authorCan, Behzat
dc.contributor.authorAtilgan, Remzi
dc.contributor.authorPala, Sehmus
dc.contributor.authorKuloglu, Tuncay
dc.contributor.authorKiray, Sule
dc.contributor.authorIlhan, Nevin
dc.date.accessioned2024-07-12T21:46:10Z
dc.date.available2024-07-12T21:46:10Z
dc.date.issued2018en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractAim: The aim of this study was to examine the effect of amifostine on cellular injury in the ovarian tissue induced by hysterosalpingography (HSG). Methods: In total, forty 4-month old female Wistar Albino rats were assigned into 8 groups. Each group contained 5 rats. Group 1 (G1): rats were decapitated without any procedure. Group 2 (G2): rats were decapitated after 3 hours of total body irradiation. Group 3 (G3): rats were decapitated 3 hours after HSG procedure. Group 4 (G4): rats were decapitated 3 hours after HSG procedure performed 30 min after receiving amifostine 200 mg/kg intraperitoneally. Group 5 (G5): rats were decapitated after 1 month without any procedure. Group 6 (G6): rats were decapitated after 1 month of total body irradiation. Group 7 (G7): rats were decapitated 1 month after HSG procedure. Group 8 (G8): rats were decapitated 1 month after HSG procedure performed 30 min after receiving amifostine 200 mg/kg intraperitoneally. After rats were decapitated under general anesthesia in all groups, blood samples were obtained and bilateral ovaries were removed. One of the ovaries was placed in 10% formaldehyde solution for histological germinal epithelial degeneration, apoptosis and proliferating cell nuclear antigen scoring. The other ovary and blood sera were stored at -80 degrees C. TNF-alpha, total antioxidant status, total oxidant status, and malondialdehyde levels were studied in tissue samples and anti-mullerian hormone levels in blood samples. Results: At the end of the first month, there was significant ovarian germinal epithelium degeneration. Proliferating cell nuclear antigen immunoreactivity was significantly reduced in all other groups when compared with G1 and G5. Conclusion: In conclusion, amifostine could significantly reduce the ovarian cellular injury induced by HSG.en_US
dc.description.sponsorshipFirat University [15.10.2014-TF.14.58]en_US
dc.description.sponsorshipThe present study was funded by Firat University, Scientific Research Grant (Project number: 15.10.2014-TF.14.58).en_US
dc.identifier.doi10.2147/DDDT.S156757
dc.identifier.endpage1500en_US
dc.identifier.issn1177-8881
dc.identifier.pmid29872271en_US
dc.identifier.scopus2-s2.0-85047831950en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1491en_US
dc.identifier.urihttps://dx.doi.org/10.2147/DDDT.S156757
dc.identifier.urihttps://hdl.handle.net/20.500.12415/7902
dc.identifier.volume12en_US
dc.identifier.wosWOS:000433209200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherDOVE MEDICAL PRESS LTDen_US
dc.relation.ispartofDRUG DESIGN DEVELOPMENT AND THERAPYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00662
dc.subjecthysterosalpingographyen_US
dc.subjectamifostineen_US
dc.subjectovarian damageen_US
dc.subjectradiationen_US
dc.titleExamination of the effect of ovarian radiation injury induced by hysterosalpingography on ovarian proliferating cell nuclear antigen and the radioprotective effect of amifostine: an experimental studyen_US
dc.typeArticle
dspace.entity.typePublication

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