Molecular markers in embryo non-development: Analysis of gene expressions (Ki-67, hTERT, HIF-1α) in spent embryo culture medium
Yükleniyor...
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Licensee MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
An embryo culture medium is a specialized set of ambient conditions, technological equipment, and nutrients that embryos require to grow properly. We aimed to investigate the Ki-67, hTERT, and HIF-1α gene expression differences between developing and non-developing embryos in spent embryo culture medium. Ki-67, hTERT, and HIF-1α gene expressions were determined from the spent embryo culture medium containing developing and non-developing embryos of 20 normoresponder patients admitted to the Bahçeci Umut IVF Center. An increase in hTERT gene expression (p < 0.05) and a decrease in HIF-1α gene expression (p < 0.001) were observed in mediums of developing compared to the non-developing embryos. No difference was observed in Ki-67 gene expression (p > 0.05). While there was a correlation between Ki-67 and HIF-1α genes in the non-growing group (r < 0.01); no correlation was observed in the developing group (r > 0.05). Both normoresponder groups will be similar in terms of proliferation rate. The low HIF-1α expression that observed high telomerase activity in embryo development maintains continuity and avoids mechanisms that result in cell death. A molecular study of the embryo development in patients with similar characteristics may help to understand the pathogenesis of the disease and establish a diagnosis and specific treatment.
Açıklama
Anahtar Kelimeler
Differentiation, Normoresponder, Non-invasive, Proliferation, Telomerase, Spent Embryo Culture Medium
Kaynak
Cells
WoS Q Değeri
Scopus Q Değeri
Cilt
13
Sayı
24
Künye
Kılıç, N. Ö. ve diğerleri. (2024). Molecular markers in embryo non-development: Analysis of gene expressions (Ki-67, hTERT, HIF-1α) in spent embryo culture medium, Cells, Licensee MDPI, 13(24), s.1-12.
