Molecular markers in embryo non-development: Analysis of gene expressions (Ki-67, hTERT, HIF-1α) in spent embryo culture medium

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dc.authorid0009-0002-5710-257X
dc.authorid0000-0002-1441-4533
dc.authorid0000-0003-3771-3277
dc.authorid0000-0003-4478-7514
dc.authorid0000-0003-3251-1043
dc.contributor.authorKılıç, Nergis Özlem
dc.contributor.authorKütük, Duygu
dc.contributor.authorÖner, Çağrı
dc.contributor.authorAslan Öztürk, Senem
dc.contributor.authorSelam, Belgin
dc.contributor.authorÇolak, Ertuğrul
dc.date.accessioned2025-01-08T13:00:50Z
dc.date.available2025-01-08T13:00:50Z
dc.date.issued2024
dc.departmentFakülteler, Tıp Fakültesi
dc.description.abstractAn embryo culture medium is a specialized set of ambient conditions, technological equipment, and nutrients that embryos require to grow properly. We aimed to investigate the Ki-67, hTERT, and HIF-1α gene expression differences between developing and non-developing embryos in spent embryo culture medium. Ki-67, hTERT, and HIF-1α gene expressions were determined from the spent embryo culture medium containing developing and non-developing embryos of 20 normoresponder patients admitted to the Bahçeci Umut IVF Center. An increase in hTERT gene expression (p < 0.05) and a decrease in HIF-1α gene expression (p < 0.001) were observed in mediums of developing compared to the non-developing embryos. No difference was observed in Ki-67 gene expression (p > 0.05). While there was a correlation between Ki-67 and HIF-1α genes in the non-growing group (r < 0.01); no correlation was observed in the developing group (r > 0.05). Both normoresponder groups will be similar in terms of proliferation rate. The low HIF-1α expression that observed high telomerase activity in embryo development maintains continuity and avoids mechanisms that result in cell death. A molecular study of the embryo development in patients with similar characteristics may help to understand the pathogenesis of the disease and establish a diagnosis and specific treatment.
dc.identifier.citationKılıç, N. Ö. ve diğerleri. (2024). Molecular markers in embryo non-development: Analysis of gene expressions (Ki-67, hTERT, HIF-1α) in spent embryo culture medium, Cells, Licensee MDPI, 13(24), s.1-12.
dc.identifier.doi10.3390/cells13242093
dc.identifier.endpage12
dc.identifier.issn2073-4409
dc.identifier.issue24
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/20.500.12415/13216
dc.identifier.volume13
dc.language.isoen
dc.publisherLicensee MDPI
dc.relation.ispartofCells
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDifferentiation
dc.subjectNormoresponder
dc.subjectNon-invasive
dc.subjectProliferation
dc.subjectTelomerase
dc.subjectSpent Embryo Culture Medium
dc.titleMolecular markers in embryo non-development: Analysis of gene expressions (Ki-67, hTERT, HIF-1α) in spent embryo culture medium
dc.typeArticle
dspace.entity.typePublication

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