Comparison of miRNA expressions among benign, premalignant and malignant lesions of the larynx: could they be transformation biomarkers?

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dc.authoridTunçturk, Fatma Ruya/0000-0002-0578-345Xen_US
dc.contributor.authorTunçtürk, Fatma Ruya
dc.contributor.authorAkalin, Ibrahim
dc.contributor.authorUzun, Lokman
dc.contributor.authorZenginkinet, Tulay
dc.date.accessioned2024-07-12T21:37:58Z
dc.date.available2024-07-12T21:37:58Z
dc.date.issued2021en_US
dc.department[Belirlenecek]en_US
dc.description.abstractBackground The malignancy potential of the laryngeal lesions are one of the major concerns of the surgeons about choosing the treatment options, forming surgical margins, deciding the follow-up periods. Finding a biomarker to overcome these concerns are ongoing challenges and recently microRNAs (miRNAs) are attributed as possible candidates since they can regulate gene expressions in the human genome. The objective of our study was to investigate their capability as a transformation biomarker for malignant laryngeal lesions. Materials and methods We investigated mature miRNA expressions in paraffin-embedded surgical specimens of human laryngeal tissues grouped as benign, premalignant or malignant (n = 10 in each). miRNA profiling was carried out by quantitative Real-Time polymerase chain reaction (RT-qPCR) and data were analyzed according to fold regulation. Results Our results demonstrated that 9 miRNAs were upregulated as the lesions become more malignant. Among them Hs_miR-183_5p, Hs_miR-155_5p, and Hs_miR-106b_3p expressions were significantly 4.16 (p = 0.032), 2.72 (p = 0.028) and 3.01 (p = 0.022) fold upregulated respectively in premalignant lesions compared to the benign lesions. Moreover, their expressions were approximately 2.76 fold higher in the malignant group than in the premalignant group compared to the benign group. Besides them, significant 7.57 (p = 0.036), 4.45 (p = 0.045) and 5.98 (p = 0.023) fold upregulations of Hs_miR-21_5p, Hs_miR-218_3p, and Hs_miR-210_3p were noticed in the malignant group but not in the premalignant group when compared to the benign group, respectively. Conclusion MiRNAs might have important value to help the clinicians for their concerns about the malignancy potentials of the laryngeal lesions. Hs_miR-183_5p, Hs_miR-155_5p, and Hs_miR-106b_3p might be followed as transformation marker, whereas Hs_miR-21_5p, Hs_miR-218_3p, and Hs_miR-210_3p might be a biomarker prone to malignancy.en_US
dc.description.sponsorship[T-UZM-2015-668]; [TSG-2013-333]en_US
dc.description.sponsorshipThe research is supported by the Research Fund of the institution. Project Numbers: T-UZM-2015-668 and TSG-2013-333. The fee of miRNeasy Mini Kit (50), miScript II RT Kit (12), miScript Primer Assay (200 rxn), miScript SYBR Green PCR Kit (400rxn) was covered by the funding.en_US
dc.identifier.doi10.1186/s40463-021-00497-y
dc.identifier.issn1916-0216
dc.identifier.issue1en_US
dc.identifier.pmid33640023en_US
dc.identifier.scopus2-s2.0-85101855419en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1186/s40463-021-00497-y
dc.identifier.urihttps://hdl.handle.net/20.500.12415/6994
dc.identifier.volume50en_US
dc.identifier.wosWOS:000622711000001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherBmcen_US
dc.relation.ispartofJournal of Otolaryngology-Head & Neck Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY04336
dc.subjectMirnaen_US
dc.subjectLarynxen_US
dc.subjectCarcinomaen_US
dc.subjectLeukoplakiaen_US
dc.subjectHyperplasiaen_US
dc.subjectBiomarkeren_US
dc.titleComparison of miRNA expressions among benign, premalignant and malignant lesions of the larynx: could they be transformation biomarkers?en_US
dc.typeArticle
dspace.entity.typePublication

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