Q-VD-OPh, a pancaspase inhibitor, reduces trauma-induced apoptosis and improves the recovery of hind-limb function in rats after spinal cord injury

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dc.authorid0000-0002-4713-4271en_US
dc.contributor.authorÇolak A.
dc.contributor.authorAntar V.
dc.contributor.authorKaraoglan A.
dc.contributor.authorAkdemir O.
dc.contributor.authorSahan E.
dc.contributor.authorÇelik Ö.
dc.contributor.authorSagmanligil A.
dc.date.accessioned2024-07-12T21:52:26Z
dc.date.available2024-07-12T21:52:26Z
dc.date.issued2009en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractBackground. Various caspases have been implicated in the development of secondary damage after spinal cord injury (SCI). Anticaspase therapy that targets only one caspase has been investigated in a variety of in vitro and in vivo studies. This study examined the neuroprotective effects of Q-VD-OPh, a pan-caspase inhibitor, in a rat model of SCI. Methods. Thirty Wistar albino rats were divided into 3 groups of 10 each: the sham-operated controls (group 1), the trauma-created controls (group 2), and the Q-VD-OPh-treated rats (group 3). An SCI (a trauma of 40 g-cm) was produced at the thoracic level (T8-T10) by the weight-drop technique. The response to injury and the neuroprotective effects of Q-VD-OPh were investigated by histopathologic examination and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) 24 hours and 5 days after trauma. The inclined plane technique of Rivlin and Tator and a modified version of Tarlov's grading scale were used to assess the functional status of the rats 24 hours, 3 days, and 5 days after injury. Results. Twenty-four hours after trauma, light microscopic examination of a specimen taken from group 2 rats revealed hemorrhage, necrosis, vascular thrombi, and edema. Group 3 tissue samples showed similar features at that time. Twenty-four hours after trauma, the mean apoptotic cell number was 4.47 ± 0.35 cells in group 2 and 1.58 ± 0.33 in group 3. Five days after injury, the mean apoptotic cell count was 4.35 ± 0.47 in group 2 and 1.25 ± 0.34 in group 3. Thus the number of TUNEL-positive cells in an injured spinal cord was greatly reduced by treatment with Q-VD-OPh. The neurologic function scores (both the inclined plane performance and motor grading scores) were significantly better in the Q-VD-OPh-treated group than in the trauma-created control group. Conclusion. The marked antiapoptotic properties of Q-VD-OPh due to the inhibition of all caspases render it a promising novel agent. A therapeutic strategy using Q-VD-OPh may eventually lead to the effective treatment of SCI in humans.en_US
dc.identifier.endpage540en_US
dc.identifier.issn1130-1473
dc.identifier.issue6en_US
dc.identifier.pmid19967318en_US
dc.identifier.scopus2-s2.0-76749113823en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage533en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8369
dc.identifier.volume20en_US
dc.identifier.wosWOS:000273100800002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherNeurocirugiaen_US
dc.relation.ispartofNeurocirugiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY03092
dc.subjectCaspaseen_US
dc.subjectQ-VD-OPhen_US
dc.subjectSCIen_US
dc.subjectSecondary damageen_US
dc.subjectSpinal cord injuryen_US
dc.subjectTUNELen_US
dc.titleQ-VD-OPh, a pancaspase inhibitor, reduces trauma-induced apoptosis and improves the recovery of hind-limb function in rats after spinal cord injuryen_US
dc.typeArticle
dspace.entity.typePublication

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