The association of piR-651 and piR-823 on metastaticand invasive characteristics of triple negative breastcancer cells
Yükleniyor...
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Group
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
PIWI-Interacting RNAs are small non-coding RNAs derived fromsingle-stranded RNAs which plays a crucial role in epigeneticregulation through transposon silencing and mRNA degrada-tion via deamination. This study aimed to inhibit piR-651 andpiR-823 in MDA-MB-231 triple-negative breast cancer cells andto explore their potential effects on healthy HUVEC cells. Non-target, anti-piR-651, and anti-piR-823 sequences were trans-fected in bothHUVEC and MDA-MB-231 cells usingLipofectamine. Proliferation and motility were assessed at 24,48, and 72 h post-transfection in both cell lines. Based on themotility findings, MDA-MB-231 cells were underwent an inva-sion assay using crystal violet staining. The expressions of Ki-67,HIF-1α, MMP-2, and MMP-9 genes were measured at 48 h,when both cell lines exhibited the most significant effects ofinhibition. The optimal time for proliferation of anti-piR-651 andanti-piR-823 transfected MDA-MB-231 cells was determined tobe at 48 h, as indicated by decreased motility and invasionassay results (p < 0.001). NeverthelessHowever, there was no sig-nificant difference in the motility and proliferation of HUVECsstransfected with anti-piR-651 and anti-piR-823 compared to thecontrol group (p > 0.05). Asides from MMP-2 in anti-piR-823transfected HUVECs and HIF-1α in anti-piR-823 transfectedMDA-MB-231 cells, gene expressions of Ki-67, HIF-1α, MMP-2,and MMP-9 were reduced in both cell lines (p < 0.001). Inhibitionof piR-651 and piR-823 decreased the survival and metastasisof cancer cells, without causing vital structural changes inhealthy cells. Future research in cancer gene therapy or geneticmodification may benefit from investigating piR-651 and piR-823 as possible inhibitors of breast cancer invasion andmetastasis.
Açıklama
Anahtar Kelimeler
Invasion, Metastasis, PIWI interacting RNA, piR-651, piR-823, Triple Negativebreast Cancer
Kaynak
Nucleosides Nucleotides & Nucleic Acids
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Öner, Ç., Köser, F. ve Çolak, E. (2024). The association of piR-651 and piR-823 on metastaticand invasive characteristics of triple negative breastcancer cells, Nucleosides Nucleotides & Nucleic Acids, Taylor & Francis Group, s.1-17.
