The association of piR-651 and piR-823 on metastaticand invasive characteristics of triple negative breastcancer cells

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dc.authorid0000-0003-3771-3277
dc.contributor.authorÖner, Çağrı
dc.contributor.authorKöser, Faruk
dc.contributor.authorÇolak, Ertuğrul
dc.date.accessioned2025-01-07T13:23:46Z
dc.date.available2025-01-07T13:23:46Z
dc.date.issued2024
dc.departmentFakülteler, Tıp Fakültesi
dc.description.abstractPIWI-Interacting RNAs are small non-coding RNAs derived fromsingle-stranded RNAs which plays a crucial role in epigeneticregulation through transposon silencing and mRNA degrada-tion via deamination. This study aimed to inhibit piR-651 andpiR-823 in MDA-MB-231 triple-negative breast cancer cells andto explore their potential effects on healthy HUVEC cells. Non-target, anti-piR-651, and anti-piR-823 sequences were trans-fected in bothHUVEC and MDA-MB-231 cells usingLipofectamine. Proliferation and motility were assessed at 24,48, and 72 h post-transfection in both cell lines. Based on themotility findings, MDA-MB-231 cells were underwent an inva-sion assay using crystal violet staining. The expressions of Ki-67,HIF-1α, MMP-2, and MMP-9 genes were measured at 48 h,when both cell lines exhibited the most significant effects ofinhibition. The optimal time for proliferation of anti-piR-651 andanti-piR-823 transfected MDA-MB-231 cells was determined tobe at 48 h, as indicated by decreased motility and invasionassay results (p < 0.001). NeverthelessHowever, there was no sig-nificant difference in the motility and proliferation of HUVECsstransfected with anti-piR-651 and anti-piR-823 compared to thecontrol group (p > 0.05). Asides from MMP-2 in anti-piR-823transfected HUVECs and HIF-1α in anti-piR-823 transfectedMDA-MB-231 cells, gene expressions of Ki-67, HIF-1α, MMP-2,and MMP-9 were reduced in both cell lines (p < 0.001). Inhibitionof piR-651 and piR-823 decreased the survival and metastasisof cancer cells, without causing vital structural changes inhealthy cells. Future research in cancer gene therapy or geneticmodification may benefit from investigating piR-651 and piR-823 as possible inhibitors of breast cancer invasion andmetastasis.
dc.identifier.citationÖner, Ç., Köser, F. ve Çolak, E. (2024). The association of piR-651 and piR-823 on metastaticand invasive characteristics of triple negative breastcancer cells, Nucleosides Nucleotides & Nucleic Acids, Taylor & Francis Group, s.1-17.
dc.identifier.doi10.1080/15257770.2024.2437037
dc.identifier.endpage17
dc.identifier.issn1525-7770
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/20.500.12415/13213
dc.language.isoen
dc.publisherTaylor & Francis Group
dc.relation.ispartofNucleosides Nucleotides & Nucleic Acids
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectInvasion
dc.subjectMetastasis
dc.subjectPIWI interacting RNA
dc.subjectpiR-651
dc.subjectpiR-823
dc.subjectTriple Negativebreast Cancer
dc.titleThe association of piR-651 and piR-823 on metastaticand invasive characteristics of triple negative breastcancer cells
dc.typeArticle
dspace.entity.typePublication

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