Sublethal concentrations of high glucose prolong mitotic arrest in a spindle assembly checkpoint activity dependent manner in budding yeast

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dc.authoridThomas, Pınar Buket/0000-0001-7627-0291en_US
dc.contributor.authorThomas, Pınar Buket
dc.contributor.authorCavusoğlu, Elif E.
dc.contributor.authorKaluc, Nur
dc.date.accessioned2024-07-12T21:37:57Z
dc.date.available2024-07-12T21:37:57Z
dc.date.issued2021en_US
dc.department[Belirlenecek]en_US
dc.description.abstractThe most successful anti-cancer drugs in clinical use interfere with cell cycle progression by inducing a mitotic arrest through activation of a conserved surveillance mechanism called the spindle assembly checkpoint (SAC). Cancer cells require much more glucose compared to normal cells to meet their energy needs due to reprogramming of their energy metabolism. Therefore, hyperglycemia may be favorable for cancer cells. Indeed, hyperglycemia is known to be closely associated with increased risk of developing several types of cancers as well as cancer related-mortality. However, the effect of hyperglycemic conditions on the mitotic arrest induced by anti-cancer drugs remains unknown. In this study, we investigated the effect of incubation in high glucose containing media on the duration of mitotic arrest induced by a SAC-activating anti-cancer drug, nocodazole, in budding yeast. We first showed that high glucose led to cell death in a dose and time dependent manner in yeast. Next, we examined the effect of sublethal concentrations of high glucose on nocodazole-induced mitotic arrest. Our data revealed that high glucose prolongs nocodazole induced mitotic arrest. Finally, we investigated whether the delay observed in exiting from nocodazole-induced mitotic arrest requires SAC activity. For this purpose, we induced a mitotic arrest that is independent of SAC activation and showed that the delay in exiting from nocodazole-induced mitotic arrest required SAC activity.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [118Z481]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with the project number: 118Z481.en_US
dc.identifier.doi10.1007/s11756-021-00912-9
dc.identifier.endpage3890en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issue12en_US
dc.identifier.scopus2-s2.0-85117772562en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage3883en_US
dc.identifier.urihttps://doi.org/10.1007/s11756-021-00912-9
dc.identifier.urihttps://hdl.handle.net/20.500.12415/6985
dc.identifier.volume76en_US
dc.identifier.wosWOS:000710858700004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY04327
dc.subjectSpindle Assembly Checkpointen_US
dc.subjectMitotic Arresten_US
dc.subjectHigh Glucoseen_US
dc.subjectCanceren_US
dc.subjectSaccharomyces Cerevisiaeen_US
dc.titleSublethal concentrations of high glucose prolong mitotic arrest in a spindle assembly checkpoint activity dependent manner in budding yeasten_US
dc.typeArticle
dspace.entity.typePublication

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