An immunoelectron microscopic study: helix pomatia lectin gold complex in breast cancer
Küçük Resim Yok
Tarih
1996
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Gazi Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Abnormal cellular glycolysation demonstrated by the bindings of HElix Pomatia Lectin (HPL) to paraffin-embedded sections has been shown in several studies to be associated with aggressive biological behavior and poor longterm prognosis in breast cancer. We investigated the relation between HPL staining of primary infiltrative duetal breast cancer and the presence of axillary lymph node (AX) metastases at the ultrastructured level by means of postembedding labeling. Breast cancer tissue fragments were fixed mild glutaraldehyde and embedded in Agar resin 100. Helix Pomatia Lectin Gold complex (HPL-GC) was chosen as a marker. A satisfactory labeling and fine structure preservation were obtained. Dense HPL binding sites were observed over the plasma membrane and the eytoplasm of cancer cells. Absorbing lectin with appropriate sugar established the specificity of binding and allowed HPL-GC to be used as a sensitive and specific reagent. The ersults of this study suggest that expression of HPL binding sites in breast cancer cells is of prognostic signifiance and may reflect the ability of tumor to invade and metastasize.
Açıklama
Anahtar Kelimeler
Helix Pomatia Lectin, Breast Cancer, Postembedding
Kaynak
Gazi Medical Journal
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
7
Sayı
3.Oca
Künye
Şeftalioğlu, A., Alabay, B., Özcan, Z., Cıncık, M. ve Kendir, B. (1996). An immunoelectron microscopic study: helix pomatia lectin gold complex in breast cancer. Gazi Medical Journal, Gazi Üniversitesi. 7 (3-1), s. 135-139.
