An immunoelectron microscopic study: helix pomatia lectin gold complex in breast cancer

dc.authorid0000-0002-0717-1756en_US
dc.contributor.authorŞeftalioğlu, Aysel
dc.contributor.authorAlabay, Belma
dc.contributor.authorÖzcan, Ziya
dc.contributor.authorCıncık, Mehmet
dc.contributor.authorKendir, Banu
dc.date.accessioned2024-07-12T21:03:52Z
dc.date.available2024-07-12T21:03:52Z
dc.date.issued1996en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractAbnormal cellular glycolysation demonstrated by the bindings of HElix Pomatia Lectin (HPL) to paraffin-embedded sections has been shown in several studies to be associated with aggressive biological behavior and poor longterm prognosis in breast cancer. We investigated the relation between HPL staining of primary infiltrative duetal breast cancer and the presence of axillary lymph node (AX) metastases at the ultrastructured level by means of postembedding labeling. Breast cancer tissue fragments were fixed mild glutaraldehyde and embedded in Agar resin 100. Helix Pomatia Lectin Gold complex (HPL-GC) was chosen as a marker. A satisfactory labeling and fine structure preservation were obtained. Dense HPL binding sites were observed over the plasma membrane and the eytoplasm of cancer cells. Absorbing lectin with appropriate sugar established the specificity of binding and allowed HPL-GC to be used as a sensitive and specific reagent. The ersults of this study suggest that expression of HPL binding sites in breast cancer cells is of prognostic signifiance and may reflect the ability of tumor to invade and metastasize.en_US
dc.identifier.citationŞeftalioğlu, A., Alabay, B., Özcan, Z., Cıncık, M. ve Kendir, B. (1996). An immunoelectron microscopic study: helix pomatia lectin gold complex in breast cancer. Gazi Medical Journal, Gazi Üniversitesi. 7 (3-1), s. 135-139.en_US
dc.identifier.endpage139en_US
dc.identifier.issn2147-2092
dc.identifier.issue3.Ocaen_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage135en_US
dc.identifier.urihttps://medicaljournal.gazi.edu.tr/index.php/GMJ/article/download/941/872/18443
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3719
dc.identifier.volume7en_US
dc.institutionauthorCıncık, Mehmet
dc.language.isoenen_US
dc.publisherGazi Üniversitesien_US
dc.relation.ispartofGazi Medical Journalen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY01066
dc.subjectHelix Pomatia Lectinen_US
dc.subjectBreast Canceren_US
dc.subjectPostembeddingen_US
dc.titleAn immunoelectron microscopic study: helix pomatia lectin gold complex in breast canceren_US
dc.typeArticle
dspace.entity.typePublication

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