Roles of adiponectin and leptin signaling-related microRNAs in the preventive effects of calorie restriction in mammary tumor development

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dc.authoridTuna, Bilge Güvenc/0000-0003-1348-1336en_US
dc.authoridÖzorhan, Ümit/0000-0002-7661-2818en_US
dc.authoridBayrak, Omer Faruk/0000-0001-7562-6604en_US
dc.authoridYılmaz, Bayram/0000-0002-2674-6535en_US
dc.authoridDOGAN, SONER/0000-0002-7762-8109en_US
dc.authoridKarabiyik, Goktug/0000-0002-0396-7626en_US
dc.contributor.authorDogan, Soner
dc.contributor.authorCicekdal, Münevver B.
dc.contributor.authorÖzorhan, Umit
dc.contributor.authorKarabiyik, Goktug
dc.contributor.authorKazan, Busra T.
dc.contributor.authorEkici, Isin D.
dc.contributor.authorYılmaz, Bayram
dc.date.accessioned2024-07-12T21:37:57Z
dc.date.available2024-07-12T21:37:57Z
dc.date.issued2021en_US
dc.department[Belirlenecek]en_US
dc.description.abstractCalorie restriction (CR) is suggested to prevent the development of mammary tumors (MTs); however, the mechanism remains to be clarified. We aimed to determine the microRNA (miRNA) profile in mice applied to 2 different CR protocols; chronic (CCR) and intermittent (ICR) and follow the MT development. In addition, the roles of miRNAs involved in adiponectin and/or leptin signaling pathways were investigated. Mice were divided into 3 groups: ad-libitum (AL), CCR, or ICR, which comprised 3 weeks of AL feeding followed by I week of 60% CR in a cyclic manner. Blood and tissue collection were performed at weeks 10,17/18, 49/50 and 81/82. Long-term CCR provided better protection compared with ICR for MT development with a delay in the MT occurrence. Adiponectin expression in mammary fat pad were significantly higher in CCR group compared with AL. Using GeneChip Array, 250 of 3195 miRNAs were differentially expressed among the dietary groups. Thirteen of 250 miRNAs were related to adiponectin and/or leptin signaling genes. Results were verified by reverse transcription polymerase chain reaction. Specifically, miR-326-3p, miR-500-3p and miR-129-5p, which are related to adiponectin and/or leptin signaling, may play important roles in the preventive effects of CR in MT development and in ageing. Thus, these miRNAs might be putative biomarkers to target for diagnostic and treatment purposes. Novelty: Type of CR and micro RNA interaction is related to ageing. miR-326-3p, miR-500-3p and miR-129-5p expression levels were differentially expressed in MT development and in ageing. The genes associated with adiponectin and/or leptin signaling pathways are regulated by certain miRNAs in the protective effects of CR.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114S429]en_US
dc.description.sponsorshipThis work was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK, 114S429). The authors thank Batuhan M. Kalkan, Burak Demir, Özan Sen, Ilker Coban, and Mustafa E. Özer for helping daily mice feeding and handling animals. The authors also thank the veterinarian and technicians at Yeditepe University Animal Facility (YUDETAM). Author contributions: S.D., B.G.T., M.P.C., O.F.B., A.K., and P.B.D. conceived the study and designed the experiments. M.B.C., U.O., G.K., B.T.K., I.D.E., I.C. and E.C.T. performed experiments and acquired data. I.D.E. performed pathological analyzes and the interpretation of the pathology results. M.B.C., S.D., B.G.T., U.O., O.F.B. and B.Y. analyzed and interpreted data. S.D., M.B.C., U.O., B.G.T. and O.F.B. wrote the first draft of the manuscript. P.B.D., A.K., B.Y., and M.P.C. critically revised the manuscript. S.D. and B.G.T. supervised the project.en_US
dc.identifier.doi10.1139/apnm-2020-1000
dc.identifier.endpage876en_US
dc.identifier.issn1715-5312
dc.identifier.issn1715-5320
dc.identifier.issue8en_US
dc.identifier.pmid33493087en_US
dc.identifier.scopus2-s2.0-85103834591en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage866en_US
dc.identifier.urihttps://doi.org/10.1139/apnm-2020-1000
dc.identifier.urihttps://hdl.handle.net/20.500.12415/6990
dc.identifier.volume46en_US
dc.identifier.wosWOS:000683798600003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherCanadian Science Publishingen_US
dc.relation.ispartofApplied Physiology Nutrition And Metabolismen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY04332
dc.subjectIntermittent Calorie Restrictionen_US
dc.subjectMirnaen_US
dc.subjectAgeingen_US
dc.subjectAdipokinesen_US
dc.subjectMammary Tumoren_US
dc.titleRoles of adiponectin and leptin signaling-related microRNAs in the preventive effects of calorie restriction in mammary tumor developmenten_US
dc.typeArticle
dspace.entity.typePublication

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