FK506 to prevent lung injury after hindlimb ischemia and reperfusion in a rat model: An electron microscopic study

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dc.authorid0000-0002-0890-2506en_US
dc.authorid0000-0001-9764-7393en_US
dc.contributor.authorAkgun, S
dc.contributor.authorTekeli, A
dc.contributor.authorIsbir, SC
dc.contributor.authorCivelek, A
dc.contributor.authorAk, K
dc.contributor.authorSirvanci, S
dc.contributor.authorArbak, S
dc.contributor.authorYaylim, I
dc.contributor.authorArsan, S
dc.date.accessioned2024-07-12T21:53:24Z
dc.date.available2024-07-12T21:53:24Z
dc.date.issued2004en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractPurpose. Hindlimb ischemia and reperfusion leads to lung injury in various animal models. We investigated the effectiveness of FK506, an immunosuppressive agent, which also modulates neutrophilic infiltration, in preventing lung injury after hindlimb ischemia and reperfusion in a rat model. Methods. Twenty-seven male Sprague-Dawley rats were randomized to received FK506 at doses of 0.3 mg/kg, 0.5 mg/kg, or 1 mg/kg body weight per day, or normal saline injections, as pretreatment, and there was also a sham group. On the 4th day, the animals were subjected to 2 h of ischemia induced by a tourniquet, followed by reperfusion of the extremities for 2 h. Lung tissue assays were performed for the lipid peroxidation product malondialdehyde (MDA) and total glutathione (GSH). Lung tissues were also examined histopathologically under light and electron microscopy. Results. The MDA levels in the study groups were significantly lower than those in the control group (P < 0.05), but the total GSH levels did not differ significantly among the groups. Histopathologically, there were no significant differences among the groups given different doses of FK506, but there was a significant difference between the control group and all the treatment groups. Conclusion. FK506 ameliorates the lung injury associated with ischemia and reperfusion of the lower limbs, and might have an inhibitory effect on the neutrophils that cause remote organ damage.en_US
dc.identifier.doi10.1007/s00595-004-2799-0
dc.identifier.endpage684en_US
dc.identifier.issn0941-1291
dc.identifier.issue8en_US
dc.identifier.pmid15290398en_US
dc.identifier.scopus2-s2.0-4043065059en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage678en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s00595-004-2799-0
dc.identifier.urihttps://hdl.handle.net/20.500.12415/8494
dc.identifier.volume34en_US
dc.identifier.wosWOS:000223021400008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofSURGERY TODAYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKY03453
dc.subjectischemia-reperfusion injuryen_US
dc.subjectlungen_US
dc.subjectextremitiesen_US
dc.titleFK506 to prevent lung injury after hindlimb ischemia and reperfusion in a rat model: An electron microscopic studyen_US
dc.typeArticle
dspace.entity.typePublication

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