Differential distribution of bFGF and bFGF receptors in human cumulus granulosa cells of poor, normal and over responder IVF patients

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dc.authorid0000-0001-5531-2587en_US
dc.authorid0000-0002-4474-7371en_US
dc.contributor.authorKarahüseyinoğlu, Serçin
dc.contributor.authorKöse Sargın, Ayşe
dc.contributor.authorVerit, F. F.
dc.contributor.authorYücel, Oğuz
dc.date.accessioned2024-07-12T21:03:50Z
dc.date.available2024-07-12T21:03:50Z
dc.date.issued2013en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractOBJECTIVE: The aim of this study is to discover the discriminations in distribution of bFGF and bFGF receptors in human cumulus cells obtained from IVF cycles performed in poor, normal and over responder patients. DESIGN: Three groups of patients were selected (poor, normal and over responders) regarding the number of oocytes obtained.Cumulus cells were stained with specific markers and observed under confocal microscope to reveal the differences. MATERIALS AND METHODS: Cumulus cells obtained from three patient groups (poor, normal and over responders, n¼20/each group) were isolated with respect to their proximity to zona pellucida, as cells at the outer most area of the follicle were isolated mechanically, while the ones neighboring the zona pellucida were isolated enzymatically.The cell suspensions were fixed in absolute acetone at -20C for 10 minutes. Cells were stained with anti-bFGF and anti-bFGF receptor.Confocal microscope images were taken under Eclipse 90i Nikon confocal microscope. RESULTS: Preliminary results showed that bFGF staining was not observed in neither cell cytoplasm nor the extracellular matrix area in all groups. However its receptors were well defined inclusively in the certain cellular membrane areas extending into cytoplasm. Although all groups showed similar staining in both outer and inner cumulus cells,the pattern was most prominent in outer cumulus cells of over responder patients. Demonstrative pictures were taken to show the specific differences. CONCLUSION: Since bFGF is known to be located in the cumulus cells and oocytes of primordial and primary follicles (to influence the development and the transition of primordial follicle), non-staining of bFGF in cumulus cells of induced patients can be expected. However the presence of bFGF receptors is important since this may demonstrate that bFGF can work through its receptors for its many activities (as antiapoptotic or mitogenic activity, etc.) if it is provided in the microenvironment of induced follicles.en_US
dc.identifier.citationKarahüseyinoğlu, S., Köse Sargın, A., Aktaş, R. G., Verit, F. F. ve Yücel, O. (2013). Differential distribution of bFGF and bFGF receptors in human cumulus granulosa cells of poor, normal and over responder IVF patients. Fertility and Sterility, Elsevier. 100(3), s. 532-533.en_US
dc.identifier.endpage533en_US
dc.identifier.issn0015-0282
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage532en_US
dc.identifier.urihttps://www.fertstert.org/article/S0015-0282(13)01073-X/fulltext#%20
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3711
dc.identifier.volume100en_US
dc.institutionauthorAktas, Ranan Gulhan
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofFertility and Sterilityen_US
dc.relation.isversionof10.1016/j.fertnstert.2013.07.294en_US
dc.relation.publicationcategoryUluslararası Konferans Öğesien_US
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.snmzKY01022
dc.titleDifferential distribution of bFGF and bFGF receptors in human cumulus granulosa cells of poor, normal and over responder IVF patientsen_US
dc.typeArticle
dspace.entity.typePublication

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