Inhibition of voltage-gated potassium channels affect expressions of miR-126 and miR-126*in breast cancer cell lines

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dc.contributor.authorCosan, Turgut D
dc.contributor.authorSoyocak, A.
dc.contributor.authorOner, C.
dc.date.accessioned2024-07-12T21:43:21Z
dc.date.available2024-07-12T21:43:21Z
dc.date.issued2020en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractAIM: We aimed to determine the possible correlation between voltage-gated potassium channels and micro RNAs in breast cancer and metastatic breast cancer cells. METHOD: Kv1.3 and Kv10.1 channels were inhibited by specific siRNAs using a lipofectamine-based transfection in MCF-7 and MDA-MB-231 cells. After transfection, total RNA was isolated, and then miR-126 and miR-126* expressions were observed using RT-PCR. RESULTS: There was a negative correlation between Kv channels and miRNAs according to the characteristics of the breast cancer cells. The inhibition was observed not only in Kv1.3 but also in Kv10.1 in MCF-7 cells, and miR-126 and miR-126* expressions were downregulated compared to the control group (p < 0.001). The inhibition of these channels in MDA-MB-231 cells caused an upregulation of miR-126 and miR-126* expressions (p < 0.001). CONCLUSION: The miR-126 and miR-126* expressions differed according to benign and malign breast cancer cell lines. Furthermore, we found that miR-126/126* may interact with Kv1.3 and Kv10.1 voltage-gated potassium channels. Our study suggests and indicates the relationship between Kv channels and miRNAs in breast cancer cells.en_US
dc.identifier.endpage116en_US
dc.identifier.issn1336-0345
dc.identifier.issue2en_US
dc.identifier.pmid32115962en_US
dc.identifier.scopus2-s2.0-85080840671en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage111en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12415/7588
dc.identifier.volume121en_US
dc.identifier.wosWOS:000508930100002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherCOMENIUS UNIVen_US
dc.relation.ispartofCOMENIUS UNIVen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsCC0 1.0 Universal*
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.snmzKY00548
dc.subjectINDUCED APOPTOSISen_US
dc.subjectPROLIFERATIONen_US
dc.subjectMICRORNASen_US
dc.subjectETHERen_US
dc.subjectLYMPHOCYTEen_US
dc.subjectRESISTANCEen_US
dc.subjectMIGRATIONen_US
dc.subjectONCOGENEen_US
dc.titleInhibition of voltage-gated potassium channels affect expressions of miR-126 and miR-126*in breast cancer cell linesen_US
dc.typeArticle
dspace.entity.typePublication

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