Microsatellite instability and methylation of the DNA mismatch repair genes in head and neck cancer
Küçük Resim Yok
Tarih
2006
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background Methylation in the promoter region of the DNA mismatch repair genes hMLH1 and hMSH2 and microsatellite instability at three loci were analyzed in the tumor tissue from patients with head and neck cancer. Methods Microsatellite instability and promoter methylation were investigated by PCR, denaturing-polyacrylamide gel electrophoresis and digestion with methylation-specific restriction enzymes. Results Microsatellite instability was observed in 41% of the patients. hMLH1 and hMSH2 genes were methylated in 47% and 30% of the patients, respectively. BAT25 and BAT26 instability were associated with age and histopathology, respectively. Methylation frequency of the hMLH1 gene promoter was significantly higher in patients displaying a high level of microsatellite instability. Instability at the BAT 26 and D2S123 loci were associated with the MSI-high status. Conclusions Our results indicate that microsatellite instability and modifications in the hMLH1 and hMSH2 genes are implicated in a significant proportion of the patients with head and neck cancer.
Açıklama
Anahtar Kelimeler
methylation, hMLH1, hMSH2, microsatellite instability, head and neck cancer
Kaynak
Annals of Oncology
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
17
Sayı
6
Künye
Demokan, S., Süoğlu, Y., Demir, D., Gözeler, M. ve Dalay, N. (2006). Microsatellite instability and methylation of the DNA mismatch repair genes in head and neck cancer. Annals of Oncology, Elsevier. 17(6), s. 995-999.
