Microsatellite instability and methylation of the DNA mismatch repair genes in head and neck cancer

Basit Öğe Kaydı
dc.authorid0000-0001-7914-2201en_US
dc.contributor.authorDemokan, Semra
dc.contributor.authorSüoğlu, Yusufhan
dc.contributor.authorDemir, Deniz
dc.contributor.authorGözeler, Mustafa
dc.contributor.authorDalay, Nejat
dc.contributor.authorDemir, Deniz
dc.date.accessioned2024-07-12T21:00:29Z
dc.date.available2024-07-12T21:00:29Z
dc.date.issued2006en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractBackground Methylation in the promoter region of the DNA mismatch repair genes hMLH1 and hMSH2 and microsatellite instability at three loci were analyzed in the tumor tissue from patients with head and neck cancer. Methods Microsatellite instability and promoter methylation were investigated by PCR, denaturing-polyacrylamide gel electrophoresis and digestion with methylation-specific restriction enzymes. Results Microsatellite instability was observed in 41% of the patients. hMLH1 and hMSH2 genes were methylated in 47% and 30% of the patients, respectively. BAT25 and BAT26 instability were associated with age and histopathology, respectively. Methylation frequency of the hMLH1 gene promoter was significantly higher in patients displaying a high level of microsatellite instability. Instability at the BAT 26 and D2S123 loci were associated with the MSI-high status. Conclusions Our results indicate that microsatellite instability and modifications in the hMLH1 and hMSH2 genes are implicated in a significant proportion of the patients with head and neck cancer.en_US
dc.identifier.citationDemokan, S., Süoğlu, Y., Demir, D., Gözeler, M. ve Dalay, N. (2006). Microsatellite instability and methylation of the DNA mismatch repair genes in head and neck cancer. Annals of Oncology, Elsevier. 17(6), s. 995-999.en_US
dc.identifier.endpage999en_US
dc.identifier.issn0923-7534
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage995en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0923753419435692?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3384
dc.identifier.volume17en_US
dc.institutionauthorDemir, Deniz
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofAnnals of Oncologyen_US
dc.relation.isversionof10.1093/annonc/mdl048en_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00266
dc.subjectmethylationen_US
dc.subjecthMLH1en_US
dc.subjecthMSH2en_US
dc.subjectmicrosatellite instabilityen_US
dc.subjecthead and neck canceren_US
dc.titleMicrosatellite instability and methylation of the DNA mismatch repair genes in head and neck canceren_US
dc.typeArticle
dspace.entity.typePublication

Dosyalar

Koleksiyon

Tıp Fakültesi Koleksiyonu