HYDROGEN PEROXIDE PROLONGS MITOTIC ARREST IN A DOSE DEPENDENT MANNER AND INDEPENDENTLY OF THE SPINDLE ASSEMBLY CHECKPOINT ACTIVITY IN SACCHAROMYCES CEREVISIAE

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dc.authorid0000-0003-1348-1336en_US
dc.authorid0000-0001-7627-0291en_US
dc.authorid0000-0002-3507-5083en_US
dc.contributor.authorAtalay, Pinar Buket
dc.contributor.authorAsci, Oyku
dc.contributor.authorKaya, Fatih Oner
dc.contributor.authorTuna, Bilge Guvenc
dc.date.accessioned2024-07-12T21:44:12Z
dc.date.available2024-07-12T21:44:12Z
dc.date.issued2017en_US
dc.departmentMaltepe Üniversitesien_US
dc.description.abstractOxidative stress and chromosome missegregation are important factors that are linked to aneuploidy. A major reason for chromosome missegragation is the inappropriate activity of the spindle assembly checkpoint (SAC), a conserved surveillance mechanism that monitors the state of kinetochore-microtubule attachments to ensure equal chromosome segregation in mitosis. SAC-activation induces a prolonged mitotic arrest. Mitosis is considered the most vulnerable cell cycle phase to several external signals, therefore increasing the time cells spent in this phase via mitotic arrest induction by SAC-activating agents is favorable for cancer therapy. Cancer cells also display elevated oxidative stress due to abnormally high production of reactive oxygen species (ROS). However, the effect of increased oxidative stress on the duration of mitotic arrest remains largely unknown. In this study, we investigated the effect of H2O2-induced oxidative stress on the mitotic arrest induced by a SAC-activating agent (nocodazole) in Saccharomyces cerevisiae. Our data suggest that oxidative stress prolongs SAC-activation induced mitotic arrest in a dose dependent manner. We, in addition, investigated the effect of H2O2 treatment on the mitotic arrest induced independently of SAC-activation by using a conditional mutant (cdc23) and showed that the effect of H2O2-induced oxidative stress on mitotic arrest is independent of the SAC activity.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114S094]en_US
dc.description.sponsorshipWe thank Dr. Daniel J. Burke (North Carolina State University, College of Sciences, Department of Biological Sciences) for kindly sending us the S. cerevisiae strains. This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with the project number: 114S094.en_US
dc.identifier.doi10.1556/018.68.2017.4.12
dc.identifier.endpage489en_US
dc.identifier.issn0236-5383
dc.identifier.issn1588-256X
dc.identifier.issue4en_US
dc.identifier.pmid29262707en_US
dc.identifier.scopus2-s2.0-85042104917en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage477en_US
dc.identifier.urihttps://dx.doi.org/10.1556/018.68.2017.4.12
dc.identifier.urihttps://hdl.handle.net/20.500.12415/7695
dc.identifier.volume68en_US
dc.identifier.wosWOS:000418558300012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherAKADEMIAI KIADO RTen_US
dc.relation.ispartofACTA BIOLOGICA HUNGARICAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00023
dc.subjectSpindle assembly checkpointen_US
dc.subjectOxidative stressen_US
dc.subjectMitotic arresten_US
dc.subjectCanceren_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.titleHYDROGEN PEROXIDE PROLONGS MITOTIC ARREST IN A DOSE DEPENDENT MANNER AND INDEPENDENTLY OF THE SPINDLE ASSEMBLY CHECKPOINT ACTIVITY IN SACCHAROMYCES CEREVISIAEen_US
dc.typeArticle
dspace.entity.typePublication

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