Toxicity of cadmium based and cadmium free quantum dots in primary cultured zebrafish hepatocytes
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Quantum Dots (QDs) are one of the most important nanomaterials in nanotechnology owing to their unique physicochemical properties. A conventional QD contains Cd2+ in its core and in order to reduce its toxicity Cd-core/ZnS-shell and Cd-free QDs were produced. However, ZnS shell was not found as protective as predicted. Also, still not much is known about the toxicity of Cd-free QD. In this paper, we evaluated and compared the cytotoxicity and genotoxicity of three types of QDs (Cd-based core, Cd-based core/shell, and Cd-free QDs) in primary cultured zebrafish hepatocytes. We show that Cd-based QDs significantly decreased cell viability whereas Cd-Free QDs caused a significant increase in cell viability in 24h. Moreover, ZnS shell reduced Cd2+ ion release into the cell but did not effectively prevent the cell from cytotoxic or genotoxic effects of the QD. Although the effects of Cd-based and Cd-Free QDs on cell viability were different, they both induced reactive oxygen species (ROS) production, DNA strand breaks and Rad51 downregulation in primary cultured zebrafish hepatocytes, revealing that they induce oxidative stress in these cells. To the best of our knowledge, this is the first report on the comparative toxicity evaluation of Cd-based with core and core/shell type, Cd-free QDs and CdCF by assessing ROS production, DNA damage and Rad51 expression. © 2020 Parlar Scientific Publications. All rights reserved.
Açıklama
Anahtar Kelimeler
Cadmium, Cadmium Free Quantum Dots, Comet, Rad51, Zebrafish
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
29
Sayı
7A
