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Comparison of the rates for reaching the blastocyst stage between normal and abnormal pronucleus embryos monitored by a time-lapse system in IVF patients
(Erkan Mor, 2021) Uzun, Kübra Nur; Cıncık, Mehmet; Selam, Belgin; Takmaz, Özgüç; Uyar, Esra
Objective: To compare the rates of blastocyst stage development between embryos fertilized after one (MPN) or more than two pronucleus (PN) (3PN, 4PN-multiPN) with those after 2PN in the same patients. Material and Methods: The embryos of patients who had both abnormal PN (MPN, 3PN or 4PN) and normal fertilized (2PN) embryos after intracytoplasmic sperm injection (ICSI) fertilization, were followed with a time-lapse system following the ICSI procedure. The rates of reaching the blastocyst stage were compared between normal and abnormally fertilized embryos. Results: One thousand eight hundred and twenty oocytes were collected from 140 patients and 1280 (70.3%) of them were fertilized. MPN, 2PN and 3PN, 4PN (multiPN) ratios of the embryos in the pronuclear stage were 11.4%, 83.13% and 5.47%, respectively. The rates of reaching the blastocyst stage among these embryos were 17.1%, 60.8% and 42.8% for MPN, 2PN and multiPN, respectively. The proportion reaching blastocyst development was significantly higher following 2PN compared to those after MPN and multiPN (p<0.05). Embryos developing after multiPN had significantly higher rates of reaching the blastocyst stage compared to those after MPN (p<0.01). Conclusion: The majority of abnormally pronucleated embryos arrest without reaching the blastocyst stage. MultiPN embryos have a higher rate of blastocyst development than MPN embryos. (J Turk Ger Gynecol Assoc 2021; 22: 120-6)
Early compaction might be a parameter to determine good quality embryos and day of embryo transfer in patients undergoing intracytoplasmic sperm injection
(Springer Nature, 2022) Sayan, Sena; Selam, Belgin
Introduction: Compaction is the first event in embryo morphogenesis. Blastocyst transfer on day five or six has been widely performed in the last decade. We investigated the clinical value of early compaction on day three for evaluation of the transferred embryo quality and pregnancy. Methods: Four hundred patients with female factor infertility and 776 fresh embryo transfers were included. Two groups were formed: Early compaction group had embryo transfer with at least one day-three embryo exhibiting early compaction. Transferred embryos without early compaction comprised the control group. Embryo transfer was performed on day three or five after the assessment of embryo compaction by a time-lapse technology system. Each patient underwent only a single cycle of embryo transfer. We analyzed fertilization, pregnancy, and live birth rates. Results: We detected significantly higher numbers of the retrieved oocytes, metaphase II (MII) oocytes, and fertilized oocytes in the early compaction group. Moreover, the transfer of the early compacting embryos on day three resulted in higher pregnancy and live birth rates. Conclusion: Our data suggest that early compaction might be a factor to determine good quality embryos and embryo transfer day.
Effects of antioxidants on motility and DNA integrity in frozen-thawed sperm
(Maltepe Üniversitesi, 2020) Selam, Belgin
Aims: This study aims to investigate whether the addition of the antioxidant complex including taurine, ascorbic acid, and glutathione into the cryopreservation medium affects the damage to sperm during the freezing process. Material and Methods: Ejaculate samples of patients who applied for semen analysis to the Assisted Reproduction Unit of Private Adatip Hospital were used. Fresh samples were analyzed for standard semen quality parameters according to the World Health Organization guidelines. Samples within the normal range were evaluated for sperm DNA fragmentation using the Halosperm technique. Remaining ejaculates were washed with the gradient method before cryopreservation. Sperm samples of each patient were divided equally for freezing in a cryopreservation medium with or without the antioxidant supplementation. One month later, the samples were thawed. Post-thaw total motility and DNA fragmentation were determined for each sample. Results: Semen samples of 40 patients were analyzed. We observed decreased total motility (34.8 ±5.32 % vs. 65.5 ±6.42 %, P= 0.002) and increased sperm DNA fragmentation (52.3±5.42 % vs. 26.4±3.12 %, P= 0.002) in post-thaw semen samples following cryopreservation in comparison to fresh samples. The addition of antioxidants to the freezing medium did not have a statistically significant effect on sperm motility (38.3± 6.22 % vs. 34.8 ±5.32 %, P =0.07) and DNA damage (47.5±4.7 % vs. 52.3±5.42, P =0.08) when compared to control samples following the freezing process. Conclusions: We observed increased sperm DNA fragmentation and decreased total motility following cryopreservation. No significant improvement in sperm motility or DNA integrity was obtained after the addition of 5 µM of the antioxidants taurine, ascorbic acid, and glutathione to the freezing media.
The Effects of Glutathione on Clinically Essential Fertility Parameters in a Bleomycin Etoposide Cisplatin Chemotherapy Model
(Mdpi, 2023) Bayram, Hale; Dönmez Çakıl, Yaprak; Sitar, Mustafa Erinc; Demirel, Gamze; Selam, Belgin; Cincik, Mehmet
Chemotherapeutic agents used in the treatment of testicular cancer cause damage to healthy tissues, including the testis. We investigated the effects of glutathione on sperm DNA integrity and testicular histomorphology in bleomycin etoposide cisplatin (BEP) treated rats. Twelve-week-old male rats of reproductive age (n = 24) were randomly divided into three groups, the (i) control group, (ii) BEP group, and (iii) BEP+ glutathione group. Weight gain increase and testes indices of the control group were found to be higher than that of the BEP group and BEP+ glutathione group. While the BEP treatment increased sperm DNA fragmentation and morphological abnormalities when compared to the control group, GSH treatment resulted in a marked decrease for both parameters. Moreover, BEP treatment significantly decreased serum testosterone levels and sperm counts in comparison to the control group, yet this reduction was recovered in the BEP+ glutathione treated group. Similarly, seminiferous tubule epithelial thicknesses and Johnsen scores in testicles were higher in the control and BEP+ glutathione groups than in the BEP-treated group. In conclusion, exogenous glutathione might prevent the deterioration of male reproductive functions by alleviating the detrimental effects of BEP treatment on sperm quality and testicular histomorphology.
Fertility following spinal cord injury
(ASRM, 2006) Cıncık, Mehmet; Akar, M. Erman; Bedir, S.; Selam, Belgin; Coban, H.; Ergür, A.R.
OBJECTIVE: To investigate the fertility of patients with spinal cord injuries (SCI) and their partners. DESIGN: Retrospective study. MATERIALS AND METHODS: Between April 2004 and April 2006, electroejaculation method was used to procure semen from 12 patients with anejaculation in 14 cycles. Sperm quality of the ejaculate was evaluated. All patients received concomitant pituitary down regulation with GnRH-agonist (Lupron, 0.1 mg sc) beginning in the midluteal phase of the preceding cycle. GnRH agonist was continued until the day of the HCG injection. Dose of recombinant FSH was individually adjusted every 1-3 days thereafter based on the results of serum E2 determinations. Routine IVF /ICSI procedures were performed. Oocytes were retrieved 34 hours after hCG injection. RESULTS: Semen was retrieved in all of the IVF/ICSI cycles. Embryo transfer was cancelled in three cycles because there was no fertilization. Mean age of the male patients was 28.4 6.1 (range 22-31). Mean number of grade 1 embryos on day 3 were 3.4 2 (range 1-10). Pregnancy rate per cycle was 21%. CONCLUSION: Semen for fertility purposes can be effectively obtained in patients with anejaculation using electroejaculation. Research may be focused on increasing sperm quality for these patients during assisted reproductive techniques.
ICSI sonrası erken dönem ß-hCG artış oranın 35 yaş altı ve üzeri kadınlarda gebelik prognozunu öngörmedeki rolü
(T.C. Sağlık Bakanlığı Ankara Şehir Hastanesi, 2022) Uzunhasanoğlu, Özge; Dönmez Çakıl, Yaprak; Selam, Belgin; Cincik, Mehmet
Amaç: İlk trimester maternal serum belirteçlerinden olan ?-human koryonik gonadotropin (?-hCG), gebeliğin erken döneminde hızla artar. Birçok çalışma, ?-hCG değerinin artma seyrinin gebelik sonucunu öngörmede yararlı olduğunu bildirmiştir. Çalışmamızda intrasitoplazmik sperm enjeksiyonu (ICSI) işlemlerini takiben erken dönem ?-hCG artış oranının, maternal yaşa bağlı olarak gebelik prognozunu öngörmedeki yerinin değerlendirilmesi ve bu sayede, bu yaş gruplarında gelişebilecek kimyasal gebelik, abortus gibi olguların daha erken ve hassas yönetimi ve takibi için faydalı olup olamayacağının araştırılması amaçlanmıştır. Gereç ve Yöntemler: ICSI tedavisi sonrası gebelik oluşan ve ß-hCG değerleri embriyo transferinden 10 ve 12 gün sonra ölçülmüş 1977 hastanın verileri retrospektif olarak incelenmiştir. Hastalar 35 yaş altı ve üzeri olarak iki gruba ayrılarak, ß-hCG artış oranları, kimyasal gebelik, klinik gebelik, abortus, ektopik gebelik, devam eden gebelik ve canlı doğum olgularında karşılaştırılmıştır. Anlamlılık p<0,05 düzeyinde değerlendirilmiştir. Bulgular: Her iki gün elde edilen ölçümler ve ß-hCG artış oranı 35 yaş altı hastalarda daha yüksek bulunmuştur (sırasıyla: p=0,001; p=0,001; p=0,006). Her iki yaş grubunda da kimyasal gebelik olgularında ß-hCG artış oranı daha düşük izlenmiştir (<35 yaş: p=0,001; ?35 yaş: p=0,001). Klinik gebelik, abortus, ektopik gebelik, devam eden gebelik ve canlı doğum olgularında, ß-hCG artış oranı karşılaştırıldığında, istatistiksel olarak anlamlı farklılık saptanmamıştır. Sonuç: Artan maternal yaş ile birlikte ?-hCG artış oranı azalmıştır. Hem 35 yaş altı hem de üzerindeki hastalarda, ß-hCG artış oranı, kimyasal gebelik olgularında diğer gebelik gruplarına göre daha düşük izlenmektedir. Erken dönem ?-hCG artış oranı, özellikle kimyasal gebelik olgularının daha erken yönetimi ve hassas takibi için faydalı olabilir.
The impact of oocyte denudation without a pre-incubation on intracytoplasmic sperm injection outcomes
(Gülhane Faculty of Medicine, 2022) Kanaat, Şenay; Selam, Belgin; Düzgün, Zehra Beyza; Cincik, Mehmet; Dönmez Çakıl, Yaprak
Aims: Debate over the optimal timing of oocyte denudation following oocyte retrieval continues. Multinucleation has been associated with lower embryo quality and implantation rate. Defining oocyte characteristics might increase the chances of better embryo selection. This study aimed to investigate the laboratory and clinical outcomes of intracytoplasmic sperm injection when the oocytes were denuded immediately after oocyte retrieval. Methods: A prospective randomized sibling-oocyte study was performed. The participants were under 40 years of age with more than 5 oocytes. Patients with male factors were excluded. The study and control groups were formed by simple randomization. The study group underwent oocyte denudation immediately. In the control group, oocytes were routinely incubated in an equilibration solution for 2 h until the removal of the cumulus cells. Outcome measures were normal fertilization (two pronuclei, 2PN), multinucleation rate, the proportion of good quality embryos, blastocyst formation, and pregnancy rate. Results: A total of 792 oocytes were collected from 54 participants (mean age: 30.6±3.7 years). The fertilization rate was higher in the study group (n=209 in 376) compared with the controls (n=201 in 416) (55.6% vs. 48.3%, p=0.041). The multinucleation rate (6.6% vs. 4.3%, p=0.150), proportion of grade 1 embryos on day 3 (48.7% vs. 43%, p=0.112) and day 5 (27.7% vs. 23.8%, p=0.214), the proportion of embryos reaching blastocyst stage (34.0% vs. 28.1%, p=0.072), and the pregnancy rates (78.6% vs. 71.4%, p=1.000) were similar. Conclusions: Our results demonstrated that immediate removal of cumulus cells does not have any negative impact on intracytoplasmic sperm injection outcomes.
İnsan embriyolarında fragmantasyon oluşumuna karşı kumulus hücre kültürü etkisi
(Medical Network Klinik Bilimler ve Doktor, 2005) Korkmaz, Cem; Akar, Münire; Selam, Belgin; Baykal, Barış
Amaç: Çalışmamızda, kumulus hücre kültürünün insan embriyolarında fragmantasyon oranına etkilerini araştırmayı amaçladık. Gereç ve Yöntem: Çalışma grubuna kontrollü ovaryan stimulasyonuna iyi cevap veren 28 infertil hasta dahil edildi. Rekombinant FSH ile kontrollü ovaryan hiperstimülasyon ve GnRH analoğu ile pituiter desensitizasyon uygulanımı sonrası bu hastalardan toplanan 344 matür oosite ICSI(intrasitoplazmik sperm enjeksiyonu) uygulandı. Matür oositler rast gele iki gruba ayrılarak kontrol ve çalışma grupları oluşturuldu. Çalışma grubundaki matür oositlere 72 saat boyunca kumulus kokültürü yapıldı. Bulgular: Her iki grup fragmantasyon oranlarına göre karşılaştırıldığında , kumulus hücre kültürü yapılan grupta grade III (kötü kalitede) olan embriyolardaki fragmantasyon oranı , kontrol grubuna göre istatistiksel olarak anlamlı ölçüde düşük bulundu. Sonuç: Kumulus hücre kokültürünün pratik ve kolay uygulanabilir bir yöntem olması nedeniyle, ICSI sonrasında elde edilen embriyolarda fragmantasyonun azaltılması için kullanılmasını mümkün görmekteyiz.
IVF outcomes after hysteroscopic metroplasty in patients with T- shaped uterus
(BMC, 2019) Uyar, Esra; Usal, Deniz; Selam, Belgin; Cıncık, Mehmet; Bağış, Tayfun
Background: T- shaped uterus may be associated with infertility and adverse pregnancy outcomes. Hysteroscopic metroplasty may improve the reproductivity for these cases. To our knowledge, there is no data in literature about the clinical consequences of in vitro fertilization (IVF) in patients undergoing hysteroscopic metroplasty for T-shaped uterus. The principal objective of the current study is to assess the impact of hysteroscopic metroplasty for T-shaped uterus on the reproductive outcomes of IVF. Methods: IVF outcomes of 74 patients who underwent hysteroscopic metroplasty for T- shaped uterus and 148 patients without any uterine abnormalities and with diagnosis of unexplained infertility (control group) were retrospectively analyzed. Results: Patients in metroplasty and control groups were comparable with respect to age, BMI, partner’s age and duration of infertility. Number of patients with a history of pregnancy beyond 20 weeks of gestation was significantly lower in the metroplasty group (4.1% vs 18.2%; p < 0.05). Number of previous unsuccessful cycles and percentage of patients with ?3 unsuccessful IVF cycles (35.1% vs 17.6%; p < 0.05) were significantly higher in the metroplasty group. There were no significant differences in the reproductive outcomes such as the pregnancy rate, clinical pregnancy or live birth rate between the metroplasty and control groups. There were non-significant trends for higher rates of miscarriage (18.8% vs 8%, p > 0.05) and biochemical pregnancy (20.0% vs 10.7%, p > 0.05) in the metroplasty group compared to the control group. Conclusions: Reproductive results of the IVF cycles after hysteroscopic correction of T-shaped uterus were comparable to those of the patients without any uterine abnormalities and with diagnosis of unexplained infertility. Hysteroscopic metroplasty may contribute to improved IVF outcomes in patients with T-shaped uterus
Mikro akışkan çip, yüzdürme (swim up) ve gradient yöntemleri kullanılarak hazırlanan sperm örneklerinde DNA fragmantasyon oranlarının karşılaştırılması
(Maltepe Üniversitesi Tıp Fakültesi, 2018) Murtaz, Tuğçe; Öztürk, Güler; Cincik, Mehmet; Selam, Belgin
Amaç: Sperm hazırlama yöntemleri, yardımlı üreme tekniklerinin sonuçlarını önemli ölçüde etkiler. Sperm yıkama işlemi, fertilizasyon için zararlı olabilecek hücrelerin ayrıştırır ve daha hareketli ve morfolojik olarak iyi spermlerin elde edilmesini sağlar. Geleneksel sperm yıkama teknikleri, santrifüjlü ve santrifüjsüz swim-up ve santrifüjlü gradient yöntemleridir. Sperm DNA fragmantasyonu, erkek infertilitesi için önerilen faktörlerden biridir. Sperm yıkama yöntemleri DNA fragmantasyonu oranlarını etkileyebilir. Bu çalışmanın amacı, geleneksel sperm yıkama yöntemleri ile yeni bir yöntem olan mikro akışkan çip yöntemi arasında DNA hasarı oranlarını kıyaslamaktır. Gereç ve Yöntem: Çalışma grubu 14 gönüllü erkekten oluşmaktadır. Alınan her semen örneği 3’e ayrılarak gradient, santrifüjsüz swim up ve mikro akışkan çip yöntemleriyle ayrı ayrı yıkandı. Her gruptaki sperm DNA fragmantasyon oranları TUNEL (terminal deoksinükleotidil transferaz-aracılı dUTP işaretleme) testi ile karşılaştırıldı. Bulgular: DNA fragmantasyonlu TUNEL pozitif sperm oranları, gradient, santrifüjsüz swim up ve mikro akışkan çip yöntemleri için sırasıyla %12, %4 ve %4,5 izlendi. Gradient yöntemi ile sperm DNA fragmantasyon oranları, diğer iki yöntemle karşılaştırıldığında istatistiksel olarak anlamlı yüksek bulundu (p<0.002). Swimup ile mikro akışkan çip yöntemleri karşılaştırıldığında DNA fragmantasyonu açısında istatistiksel olarak anlamlı bir fark izlenmedi. (p=0.583). Sonuç: Mevcut çalışmada santrifüj olmayan swim-up ve microfluidic chip yöntemlerine kıyasla gradient yöntemiyle sperm DNA fragmantasyon oranlarında artış tespit edilmiştir. Santrifüj oksidatif stresi arttırabilir ve böylece serbest radikal üretimine yol açabilir.
Rescue IVM of denuded GV- and MI-stage oocytes of premenopausal rats with oncostatin M, insulin-like growth factor I, and growth hormone
(MDPI, 2022) Akdemir, Yeşim; Selam, Belgin; Dönmez Çakıl, Yaprak; Sitar, Mustafa Erinç; Cincik, Mehmet
Immature oocytes are retrieved and matured through in vitro maturation (IVM). Maturation, fertilization rates, and embryo development via IVM are all lower than those found in vitro fertilization (IVF) cycles. We investigated the effects of oncostatin M (OSM), insulin-like growth factor-1 (IGF-I), and growth hormone (GH) in rescue IVM. A total of 111 germinal vesicle (GV) and 17 metaphase I (MI) oocytes were obtained after conventional IVF from 28 female Wistar albino rats. Denuded immature oocytes were cultured in maturation medium supplemented with OSM, IGF-1, or GH. The quantities of metaphase II (MII) oocytes matured from the GV stage were 17 of 30 (56.6%), 15 of 28 (53.5%), 10 of 30 (33.3%), and 7 of 23 (30.3%), in control, OSM, IGF-I, and GH groups, respectively. Maturation rates in control and OSM groups were higher than those in IGF-I and GH groups (p = 0.001). The quantities of MII oocytes matured from MI stage were 7 of 7 (100%), 4 of 4 (100%), 1 of 1 (100%), and 1 of 5 (20%) in control, OSM, IGF-I, and GH groups, respectively. Maturation rates from MI to MII stages in control, OSM, and IGF-I groups were higher than those in the GH group (p = 0.004). Acceptable maturation rates are observed with OSM in rat oocytes in rescue IVM.
Rescue IVM of denuded GV- and mi-stage oocytes of premenopausal rats with oncostatin M, insulin-like growth factor ı, and growth hormone
(Life, 2022) Akdemir, Yeşim; Çakıl, Yaprak Dönmez; Selam, Belgin; Sitar, Mustafa Erinç; Cıncık, Mehmet
Immature oocytes are retrieved and matured through in vitro maturation (IVM). Maturation, fertilization rates, and embryo development via IVM are all lower than those found in vitro fertilization (IVF) cycles. We investigated the effects of oncostatin M (OSM), insulin-like growth factor-1 (IGF-I), and growth hormone (GH) in rescue IVM. A total of 111 germinal vesicle (GV) and 17 metaphase I (MI) oocytes were obtained after conventional IVF from 28 female Wistar albino rats. Denuded immature oocytes were cultured in maturation medium supplemented with OSM, IGF-1, or GH. The quantities of metaphase II (MII) oocytes matured from the GV stage were 17 of 30 (56.6%), 15 of 28 (53.5%), 10 of 30 (33.3%), and 7 of 23 (30.3%), in control, OSM, IGF-I, and GH groups, respectively. Maturation rates in control and OSM groups were higher than those in IGF-I and GH groups (p = 0.001). The quantities of MII oocytes matured from MI stage were 7 of 7 (100%), 4 of 4 (100%), 1 of 1 (100%), and 1 of 5 (20%) in control, OSM, IGF-I, and GH groups, respectively. Maturation rates from MI to MII stages in control, OSM, and IGF-I groups were higher than those in the GH group (p = 0.004). Acceptable maturation rates are observed with OSM in rat oocytes in rescue IVM.
Serum and follicular fluid levels of soluble Fas and soluble Fas ligand in IVF cycles
(Elsevier, 2006) Önalan, Göğşen; Selam, Belgin; Önalan, Reşide; Ceyhan, Temel; Pabuçcu, Recai
Objective To determine follicular fluid (FF) and serum levels of soluble Fas (sFas) and soluble Fas ligand (sFasL) in patients undergoing IVF cycles. Study Design A prospective comparative study among patients with endometriosis (n = 12), infertility due to male factor (n = 12) and poor responders (n = 32) undergoing IVF cycles in Centrum IVF Clinic. Individual FF and serum samples were collected from patients during transvaginal ultrasonography-guided follicle aspiration. Patients were classified as poor responder patients undergoing IVF cycles with GnRHa, triptorelin and GnRH antagonist, cetrotide, patients with endometriosis and patients with infertility due to male factor. sFas, sFasL levels in both FF and serum samples and their correlations with clinical outcomes of IVF were measured in each study group. Results Serum and FF levels of sFas, sFasL were similar in the poor responder and male factor groups. There were no differences between the serum and FF levels of both sFas and sFasL among poor responder patients receiving either GnRH agonist or antagonist therapies. Serum levels of sFas were significantly lower in the endometriosis group compared to the male factor group. Serum and FF levels of sFas, sFasL were similar among patients with or without clinical pregnancy. Conclusion sFas and sFasL are detected in both serum and follicular fluid samples from IVF cycles, their levels are similar between poor responder and male factor groups as well as between GnRH agonist and antagonist treatment groups. These soluble apoptotic factors may not be predictive for the outcomes of IVF. Decreased serum levels of sFas, suggests increased apoptosis in endometriosis.
Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF
(Oxford Academic, 2005) Önalan, Göğşen; Selam, Belgin; Baran, Yusuf; Önalan, Reşide; Gündüz, Ufuk; Ural, Ali Uğur; Pabuçcu, Recai
BACKGROUND: There are limited data about the levels of soluble apoptotic factors and their modulation with therapeutic regimens in IVF cycles. The aim of the current study was to determine follicular fluid, and serum levels of soluble Fas (sFas) and soluble Fas ligand (sFasL) in PCOS patients undergoing IVF/ICSI cycles; also to investigate the effects of metformin on these factors and on apoptosis of luteinized granulosa cells. METHODS: We investigated the serum and follicular fluid levels of sFas and sFasL in patients with PCOS (n=28) and compared them with those of the patients with infertility due to male factor (n=12) undergoing IVF cycles. Effects of metformin therapy on these parameters and apoptosis of luteinized granulosa cells were also investigated among the patients with PCOS. RESULTS: Serum levels of sFas were significantly lower in the PCOS group compared to those in women with infertility due to male factor. Metformin therapy in PCOS patients preceding IVF cycles increased serum levels of sFas and decreased follicular fluid levels of sFasL compared to those on placebo. Follicular fluid from PCOS patients demonstrated luteinized granulosa cell DNA fragmentation in agarose gel, whereas a similar pattern was not observed among PCOS patients undergoing metformin therapy. CONCLUSION: Decreased serum levels of sFas and luteinized granulosa cell DNA fragmentation is observed in patients with PCOS undergoing IVF cycles. Metformin therapy preceding IVF demonstrates an antiapoptotic effect with increased serum levels of sFas, decreased follicular fluid levels of sFasL and prevention of luteinized granulosa cell DNA fragmentation.
Sperm selection and embryo development: a comparison of the density gradient centrifugation and microfluidic chip sperm preparation methods in patients with astheno-teratozoospermia
(MDPI, 2021) Selam, Belgin; Çağla, Güler; Melil, Süreyya; Özekici, Ümit; Dönmez Çakıl, Yaprak; Cincik, Mehmet
In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization. This prospective study aims to compare the effects of density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in patient populations with astheno-teratozoospermia. In the study, the semen samples of the patients were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were then used to fertilize mature sibling oocytes and the semen parameters and embryo development on days 3 and 5 were assessed. While the density gradient group was associated with a higher sperm concentration, motility (progressive and total) was significantly higher in the microfluidic chip group. No significant differences were observed in the fertilization rates or grade 1 (G1) and grade 2 (G2) proportions of the third-day embryos. Furthermore, while the proportions of the poor, fair and good blastocysts on day 5 did not differ significantly, excellent blastocysts (indicating high-quality embryos) were observed in a significantly higher proportion of the microfluidic chip group. When compared to the classical density gradient method, the microfluidic chip sperm preparation yielded sperm with higher motility and higher quality blastocysts at day 5; in patients with astheno-teratozoospermia.
Time-lapse (tam zamanli görüntüleme sistemi) ile takip edilen IVF sikluslarinda, teratozoospermi ile normozoospermi vakalarinin embriyo morfokinetiğinin retrospektif karşılaştırılması
(Maltepe Üniversitesi, 2019) Berberoğlu, Özge; Cıncık, Mehmet; Selam, Belgin
Amaç: Çalışmanın amacı, ICSI sikluslarında sperm morfolojisi ile fertilizasyon, gebelik oranı ve embriyo kalitesinin ilişkisini araştırmak ayrıca sperm morfolojisi ile erken yarıklanma yüzdesini karşılaştırmaktır. Gereç ve Yöntem: Reprospektif olarak planlanan çalışmaya 60 infertil çift alınmıştır. Azospermik hastalar çalışmaya dahil edilmezken kadın yaşı 40’ın altında tutulmuştur. Sperm morfolojisi hematoksilen yöntemiyle boyanan preperatlar, Kruger morfoloji kriterlerine göre değerlendirilmiştir. Sperm morfolojisi ?%4, normal kabul edilmiştir. Fertilizasyon, 16- 18. saatte 2 pronukleus olarak kabul edilirken, embriyo kalitesi embriyoların 3. günde morfolojik olarak günüyle uyumlu blastomer sayısı, blastomer büyüklüklerinin eşitliği ve fragmentasyon durumuna göre grade 1, 2, 3 olarak klasifiye edilmiştir. Çalışmada, sperm morfolojisi ile fertilizasyon, morfokinezis, embriyo kalitesi, erken yarıklanma ve gebelik oranları değerlendirildi. Bulgular: Normal morfolojili sperm oranı <%4, (n=30; çalışma grubu), ve ?%4, (n=30; kontrol grubu) olmak üzere toplam 60 hastanın verileri istatistiksel değerlendirmeye alındı. Fertilizasyon oranı, normozoospermi grubunda %53, teratozoospermi grubunda %46 bulunmuştur (p>0,01). Bu gruplar embriyo kalitesi olarak karşılaştırıldığında grade 1, 2, 3 embriyo oranlarında, gruplar arasında fark izlenmemiştir (p>0,01). Erken yarıklanma ile sperm morfolojisi arasında anlamlı bir ilişki bulunamamıştır (p>0,01). Gebelik oranları incelendiğinde sonuç, teratozoospermi grubunda %45, normozoospermi grubunda %55’dir (p>0,01). Sonuç: Bu çalışmada, sperm morfolojisi ile fertilizasyon, embriyo kalitesi, erken yarıklanma ve gebelik yüzdeleri arasında anlamlı bir ilişki görülmemiştir.

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